Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China

NWIPB OpenIR

	Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China
	Wang, Dongxia; Cao, Dong; Zong, Yuan; Li, Yun; Wang, Jinmin; Li, Zongren; Liu, Baolong
	2021
发表期刊	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
卷号	35 期号:1
摘要	The awnless trait is the favorite trait for wheat breeding in Qinghai, China, but the major gene underlying the trait is unknown. This study aimed to analyze a recombinant inbred line (RIL) population containing 112 lines by crossing common wheat varieties GY448 (awnless) and GY115 (awned) using genotyping-by-sequencing analysis. A total of 56.62 Gb of clean sequence data were generated. A major gene was identified for the awnless trait based on 101,275 single-nucleotide polymorphisms by quantitative trait locus (QTL)-seq analysis. The region from 685.50 to 709.77 Mb on chromosome 5AL contained 334 genes and 394 transcripts, including B1, the major gene controlling the awnless trait. A 25-bp indel located in the B1 promoter region has a diagnostic value for the awnless/awned genotypes in the RIL population. A total of 42 spring wheat cultivars in Qinghai, China, were analyzed. Further, 23 awnless cultivars had the alleles of the GY448 parent, while 19 awned cultivars had the alleles of the GY115 parent. B1 is crucial in the awnless trait in wheat cultivars in Qinghai, China. The findings of this study might guide the breeding of new wheat cultivars in this region.
关键词	Awnless B1 Qinghai QTL-seq wheat
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60889
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Dongxia,Cao, Dong,Zong, Yuan,et al. Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2021,35(1).
APA	Wang, Dongxia.,Cao, Dong.,Zong, Yuan.,Li, Yun.,Wang, Jinmin.,...&Liu, Baolong.(2021).Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,35(1).
MLA	Wang, Dongxia,et al."Bulked QTL-Seq identified a major QTL for the awnless trait in spring wheat cultivars in Qinghai, China".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 35.1(2021).