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Large-scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high-speed counter-current chromatography
Song, ZB; Chen, T; Wang, S; Shen, C; Ma, YM; Li, AJ; Chen, Z; Li, YL
2022
发表期刊JOURNAL OF SEPARATION SCIENCE
摘要The separation of polar compounds is challenging work due to poor retention and insufficient selectivity. In the present study, an efficient strategy for large-scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn has been established by preparative high-speed counter-current chromatography. Macroporous resin column chromatography was used for the enrichment of the polar polyphenols. However, sugar and other ultra-polar impurities were co-washed out with the targets. Liquid-liquid extraction with ethyl acetate/water (1/1, v/v) solvent system was developed to remove the ultra-polar impurities with a clearance rate of 95%. Finally, the targets were introduced to preparative high-speed counter-current chromatography for separation using ethyl acetate/n-butanol/acetic acid/water (2/7/1/10, v/v/v/v) solvent system. As a result, 191 mg of Mucic acid 1,4-lactone 5-O-gallate, 370 mg of beta-Glucogallin, 301 mg of Gallic acid, 195 mg of Mucic acid 1,4-lactone 3-O-gallate and 176 mg of Mucic acid 1,4-lactone 2-O-gallate with purity higher than 98% were obtained from 1.5 g of sample. Mucic acid 1,4-lactone 3-O-gallate, Mucic acid 1,4-lactone 3-O-gallate, and Mucic acid 1,4-lactone 2-O-gallate are isomers. The results showed that high-speed counter-current chromatography could be well developed for the separation of polar compounds from natural products.
收录类别SCIE
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/61052
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Song, ZB,Chen, T,Wang, S,et al. Large-scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2022.
APA Song, ZB.,Chen, T.,Wang, S.,Shen, C.,Ma, YM.,...&Li, YL.(2022).Large-scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE.
MLA Song, ZB,et al."Large-scale preparation of five polar polyphenols including three isomers from Phyllanthus emblica Linn. by preparative high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE (2022).
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