The Isolation and Preparation of Samwinol from Dracocephalum heterophyllum and Prevention on A beta(25-35)-Induced Neuroinflammation in PC-12 Cells

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	The Isolation and Preparation of Samwinol from Dracocephalum heterophyllum and Prevention on A beta(25-35)-Induced Neuroinflammation in PC-12 Cells
	Li, CZ; Dang, J; Lv, Y; Fang, Y; Ma, CJ; Wang, QL; Li, G
	2022
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	23 期号:19
摘要	Dracocephalum heterophyllum (D. heterophyllum) is a traditional Chinese Tibetan medicine that has been used for the treatment of lymphitis, hepatitis, and bronchitis. However, only a few selected chemical components are currently obtained from D. heterophyllum, which limits its further pharmacological applications. In this study, we have obtained samwinol from D. heterophyllum by medium- and high-pressure liquid chromatography separation for the first time. Thereafter, we investigated the protective actions of samwinol against amyloid beta protein fragment 25-35 (A beta(25-35)) induced neurotoxicity in cultured rat pheochromocytoma PC-12 cells and explored its underlying mechanisms of action. The results indicated that samwinol could increase cell viability and inhibit the production of reactive oxygen species (ROS) and mitochondria-derived ROS, as assessed by MTT assay, Giemsa staining, and flow cytometry assay. Through Western blot analysis, it was found that samwinol substantially inhibited the phosphorylation of ERK(1/2) and promoted the expression of HO-1 and Nrf2. The data obtained from molecular docking were also consistent with the above conclusions. All of these results showed that samwinol from D. heterophyllum can display significant anti-neuroinflammatory and antioxidant activities in vitro, which are associated with the suppression of ERK/AKT phosphorylation and the activation of the Nrf2/HO-1 signaling pathway. In the future, additional in-depth mechanism studies will be carried out to provide more evidence for the potential of samwinol in the treatment of Alzheimer's disease.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61092
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, CZ,Dang, J,Lv, Y,et al. The Isolation and Preparation of Samwinol from Dracocephalum heterophyllum and Prevention on A beta(25-35)-Induced Neuroinflammation in PC-12 Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(19).
APA	Li, CZ.,Dang, J.,Lv, Y.,Fang, Y.,Ma, CJ.,...&Li, G.(2022).The Isolation and Preparation of Samwinol from Dracocephalum heterophyllum and Prevention on A beta(25-35)-Induced Neuroinflammation in PC-12 Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(19).
MLA	Li, CZ,et al."The Isolation and Preparation of Samwinol from Dracocephalum heterophyllum and Prevention on A beta(25-35)-Induced Neuroinflammation in PC-12 Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.19(2022).