Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak

NWIPB OpenIR

	Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak
	Gao, X; Wang, S; Wang, YF; Li, S; Wu, SX; Yan, RG; Zhang, YW; Wan, RD; He, Z; Song, RD; Zhao, XQ; Wu, DD; Yang, QE
	2022
发表期刊	NATURE COMMUNICATIONS
卷号	13 期号:1
摘要	Wild yak (Bos mutus) and domestic yak (Bos grunniens) are adapted to high altitude environment and have ecological, economic, and cultural significances on the Qinghai-Tibetan Plateau (QTP). Currently, the genetic and cellular bases underlying adaptations of yak to extreme conditions remains elusive. In the present study, we assembled two chromosome-level genomes, one each for wild yak and domestic yak, and screened structural variants (SVs) through the long-read data of yak and taurine cattle. The results revealed that 6733 genes contained high-FST SVs. 127 genes carrying special type of SVs were differentially expressed in lungs of the taurine cattle and yak. We then constructed the first single-cell gene expression atlas of yak and taurine cattle lung tissues and identified a yak-specific endothelial cell subtype. By integrating SVs and single-cell transcriptome data, we revealed that the endothelial cells expressed the highest proportion of marker genes carrying high-FST SVs in taurine cattle lungs. Furthermore, we identified pathways which were related to the medial thickness and formation of elastic fibers in yak lungs. These findings provide new insights into the high-altitude adaptation of yak and have important implications for understanding the physiological and pathological responses of large mammals and humans to hypoxia.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61110
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Gao, X,Wang, S,Wang, YF,et al. Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Gao, X.,Wang, S.,Wang, YF.,Li, S.,Wu, SX.,...&Yang, QE.(2022).Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak.NATURE COMMUNICATIONS,13(1).
MLA	Gao, X,et al."Long read genome assemblies complemented by single cell RNA-sequencing reveal genetic and cellular mechanisms underlying the adaptive evolution of yak".NATURE COMMUNICATIONS 13.1(2022).