Specific ablation of Hippo signalling component Yap1 in retinal progenitors and Muller cells results in late onset retinal degeneration

NWIPB OpenIR

	Specific ablation of Hippo signalling component Yap1 in retinal progenitors and Muller cells results in late onset retinal degeneration
	Yang, YM; Jiang, XY; Li, X; Sun, KX; Zhu, XJ; Zhou, B
	2022
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY
卷号	237 期号:6 页码:2673
摘要	Yes-associated protein (YAP) is a major component of the Hippo pathway involved in development, growth, repair and homeostasis. Nonsense YAP1 mutations in humans result in autosomal dominant coloboma. Here, we generated a conditional knockout mouse model in which Yap1 was specifically deleted in embryonic retinal progenitor cells (RPCs) and in mature Muller cells using a Chx10-Cre driver. Our data demonstrated that the conditional ablation of Yap1 in embryonic RPCs does not prevent normal retinal development and caused no gross changes in retinal structure during embryonic and early postnatal life. Nevertheless, Yap1 deficient in retinal Muller cells in adult mice leads to impaired visual responses and extensive late-onset retinal degeneration, characterized by reduced cell number in all retinal layers. Immunofluorescence data further revealed the degeneration and death of rod and cone photoreceptors, bipolar cells, horizontal cells, amacrine cells and ganglion cells to varying degrees in aged knockout mice. Moreover, alteration of glial homeostasis and reactive gliosis were also observed. Finally, cell proliferation and TUNEL assay revealed that the broad retinal degeneration is mainly caused by enhanced apoptosis in late period. Together, this work uncovers that YAP is essential for the normal vision and retinal maintenance, highlighting the crucial role of YAP in retinal function and homeostasis.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61219
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, YM,Jiang, XY,Li, X,et al. Specific ablation of Hippo signalling component Yap1 in retinal progenitors and Muller cells results in late onset retinal degeneration[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2022,237(6):2673.
APA	Yang, YM,Jiang, XY,Li, X,Sun, KX,Zhu, XJ,&Zhou, B.(2022).Specific ablation of Hippo signalling component Yap1 in retinal progenitors and Muller cells results in late onset retinal degeneration.JOURNAL OF CELLULAR PHYSIOLOGY,237(6),2673.
MLA	Yang, YM,et al."Specific ablation of Hippo signalling component Yap1 in retinal progenitors and Muller cells results in late onset retinal degeneration".JOURNAL OF CELLULAR PHYSIOLOGY 237.6(2022):2673.