LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein-like 2, regulates tillering in switchgrass

NWIPB OpenIR

	LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein-like 2, regulates tillering in switchgrass
	Yang, RJ; Liu, WW; Sun, Y; Sun, ZC; Wu, ZY; Wang, YM; Wang, MQ; Wang, HL; Bai, SQ; Fu, CX
	2022
发表期刊	NEW PHYTOLOGIST
卷号	235 期号:2 页码:563
摘要	Strigolactones (SLs) play a critical role in regulating plant tiller number. LATERAL BRANCHING OXIDOREDUCTASE (LBO) encodes an important late-acting enzyme for SL biosynthesis and regulates shoot branching in Arabidopsis. However, little is known about the function of LBO in monocots including switchgrass (Panicum virgatum L.), a dual-purpose fodder and biofuel crop. We studied the function of PvLBO via the genetic manipulation of its expression levels in both the wild-type and miR156 overexpressing (miR156(OE)) switchgrass. Co-expression analysis, quantitative real-time polymerase chain reaction (qRT-PCR), transient dual luciferase assay, and chromatin immunoprecipitation-qPCR were all used to determine the activation of PvLBO by miR156-targeted Squamosa Promoter Binding Protein-like 2 (PvSPL2) in regulating tillering of switchgrass. PvLBOtranscripts dramatically declined in miR156(OE) transgenic switchgrass, and the overexpression of PvLBO in the miR156(OE) transgenic line produce fewer tillers than the control. Furthermore, we found that PvSPL2 can directly bind to the promoter of PvLBO and activate its transcription, suggesting that PvLBO is a novel downstream gene of PvSPL2. We propose that PvLBO functions as an SL biosynthetic gene to mediate tillering and acts as an important downstream factor in the crosstalk between the SL biosynthetic pathway and the miR156-SPL module in switchgrass.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61235
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, RJ,Liu, WW,Sun, Y,et al. LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein-like 2, regulates tillering in switchgrass[J]. NEW PHYTOLOGIST,2022,235(2):563.
APA	Yang, RJ.,Liu, WW.,Sun, Y.,Sun, ZC.,Wu, ZY.,...&Fu, CX.(2022).LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein-like 2, regulates tillering in switchgrass.NEW PHYTOLOGIST,235(2),563.
MLA	Yang, RJ,et al."LATERAL BRANCHING OXIDOREDUCTASE, one novel target gene of Squamosa Promoter Binding Protein-like 2, regulates tillering in switchgrass".NEW PHYTOLOGIST 235.2(2022):563.