Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway

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	Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway
	Wang, WD; Zhang, J; Liu, ZG; Zhu, YH; Mei, LJ; Tao, YD; Jiang, L
	2022
发表期刊	RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
卷号	48 期号:2 页码:300
摘要	Saussurea obvallata has been used as a medicinal herb to treat rheumatoid arthritis in Qinghai-Tibet Plateau. Xanthotoxol, one of the major ingredients of S. obvallata, displays anti-inflammation bioactivities according to previous studies. However, the underlying mechanism of xanthotoxol in anti-inflammation have not yet been elucidated. In this study, XT was isolated from S. obvallata for the first time and we investigated the anti-inflammatory activity and the related molecular mechanism in LPS-activated macrophages in vitro model. The cytotoxicity of XT was determined through MTT assay and flow cytometry analysis. The anti-inflammatory activities of XT were determined according to its inhibitory effects on NO, and alpha pro-inflammatory cytokines. The date showed that XT significantly reduced inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta in LPS-induced RAW264.7 cells as well as the synthesis of NO in a dose dependent manner. Moreover, xanthotoxol down-regulated the phosphorylation of NF-kappa B factors (p-P65, p-I kappa B alpha, p-IKK alpha/beta), the expressions of INOS and NFAT1 and the P65 translocation from the cytoplasm to the nucleus. These results suggested that XT exhibited significant anti-inflammatory activity in LPS-induced RAW264.7 cells model by inhibiting NF-kappa B pathway. These results might provide some explanations of the anti-inflammatory activity of S. obvallata and contribute to the further investigation and application of XT as a potential anti-inflammatory drug.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61255
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, WD,Zhang, J,Liu, ZG,et al. Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway[J]. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,2022,48(2):300.
APA	Wang, WD.,Zhang, J.,Liu, ZG.,Zhu, YH.,Mei, LJ.,...&Jiang, L.(2022).Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway.RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,48(2),300.
MLA	Wang, WD,et al."Xanthotoxol from Saussurea obvallata Attenuates LPS-Induced RAW 264.7 Cells Inflammatory Responses through NF-kappa B Pathway".RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY 48.2(2022):300.