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Bioactivity-guided isolation of the major anthocyanin from Lycium ruthenicum Murr. fruit and its antioxidant activity and neuroprotective effects in vitro and in vivo
Chen, SS; Hu, N; Wang, HL; Wu, YN; Li, GL
2022
Source PublicationFOOD & FUNCTION
Volume13Issue:6Pages:3247
AbstractLycium ruthenicum Murr. fruit (LRF) is an edible berry known for its rich anthocyanin content. Our previous study has shown that LRF-derived anthocyanins have neuroprotective effects in rats, which may be due to their effective antioxidant activity. Therefore, this study performed online HPLC-DPPH screening as a bioactivity-guided method for the preparative separation of anthocyanins from LRF. Finally, the main fraction was isolated and identified as petunidin-3,5-O-diglucoside (Pn3G5G). Pn3G5G exhibited strong antioxidant capacity during DPPH and ABTS free radical scavenge assays. Furthermore, Pn3G5G exhibited protective effects on N-epsilon-carboxymethyllysine (CML)-treated Neuro-2a cells by enhancing cell viability in a dose-dependent manner. CML-induced apoptosis was also reduced by Pn3G5G potentially by suppressing oxidative stress and inflammation. More importantly, Pn3G5G significantly improved cognitive impairment, neuroinflammation and neuronal apoptosis in d-galactose-induced aging mice. The result suggests the development of Pn3G5G as a healthcare product or a potent dietary supplement with antioxidant and neuroprotective effects.
Indexed BySCIE
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/61287
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Chen, SS,Hu, N,Wang, HL,et al. Bioactivity-guided isolation of the major anthocyanin from Lycium ruthenicum Murr. fruit and its antioxidant activity and neuroprotective effects in vitro and in vivo[J]. FOOD & FUNCTION,2022,13(6):3247.
APA Chen, SS,Hu, N,Wang, HL,Wu, YN,&Li, GL.(2022).Bioactivity-guided isolation of the major anthocyanin from Lycium ruthenicum Murr. fruit and its antioxidant activity and neuroprotective effects in vitro and in vivo.FOOD & FUNCTION,13(6),3247.
MLA Chen, SS,et al."Bioactivity-guided isolation of the major anthocyanin from Lycium ruthenicum Murr. fruit and its antioxidant activity and neuroprotective effects in vitro and in vivo".FOOD & FUNCTION 13.6(2022):3247.
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