Effect of BMP-Wnt-Nodal signal on stem cell differentiation

NWIPB OpenIR

	Effect of BMP-Wnt-Nodal signal on stem cell differentiation
	Wang, LY; Ma, RH; Jia, GX; Jian, SY; Zeng, XH; Xiong, ZF; Li, BY; Li, C
	2022
发表期刊	ZYGOTE
卷号	30 期号:1 页码:138
摘要	The generation of germ cells from embryonic stem cells in vitro has current historical significance. Western blot, qPCR, immunofluorescence and flow cytometry assays were used to investigate the differences in expression levels of totipotency and specific markers for Wnt regulation and the related signalling pathways during primordial germ cell-like cell (PGCLC) induction and differentiation. During PGCLC induction, activation of WNT3a increased the expression of NANOG, SOX2 and OCT4, but Mvh, DAZL, Blimp1, TFAP2C, Gata4, SOX17, EOMES, Brachyury and PRDM1 expression levels were significantly reduced. Inhibition of the WNT signal demonstrated the opposite effect. Similarly, inhibitors of BMP and the Nodal/Activin signal were used to determine the effect of signal pathways on differentiation. CER1 affected the Wnt signal and differentiation, but the inhibitor SB only regulated differentiation. BMP-WNT-NODAL were mainly responsible for regulating differentiation. Our results provide a reliable theoretical basis and feasibility for further clinical medical research.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61302
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, LY,Ma, RH,Jia, GX,et al. Effect of BMP-Wnt-Nodal signal on stem cell differentiation[J]. ZYGOTE,2022,30(1):138.
APA	Wang, LY.,Ma, RH.,Jia, GX.,Jian, SY.,Zeng, XH.,...&Li, C.(2022).Effect of BMP-Wnt-Nodal signal on stem cell differentiation.ZYGOTE,30(1),138.
MLA	Wang, LY,et al."Effect of BMP-Wnt-Nodal signal on stem cell differentiation".ZYGOTE 30.1(2022):138.