Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes

NWIPB OpenIR

	Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes
	Chen, T; Yang, X; Wang, S; Shen, C; Li, HM; Wei, YF; Yan, SP; Song, ZB; Yang, FM; Liu, YR; Hai, P; Li, YL
	2022
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	45 期号:2 页码:468
摘要	The separation of compounds with similar polarities is challenging. In the present study, five flavone glycosides, including two groups with similar polarities, were obtained from Dracocephalum tanguticum by three high-speed counter-current chromatography modes, including flow rate conversion mode, recycling mode, and heart-cut mode. With flow rate conversion mode, compounds 3 and 4 with similar polarities and compound 5 were separated by high-speed counter-current chromatography with ethyl acetate/methanol/water (5.0% acetic acid) (8:2:10, v/v) system. The flow rate was controlled as: 1.8 mL/min for 0-160 min, 2.2 mL/min for 160-200 min, and 2.5 mL/min for 200-400 min. However, compounds 1 and 2 with similar polarities were not separated due to the similar distributive properties. Then, a recycling and heart-cut mode were introduced to improve the separation efficiency. The heart-cut mode was introduced in the second and third cycles, and compounds 1 and 2 were well separated in the fourth cycle. Consequently, five flavone glycosides, including two groups with similar polarities were obtained and identified as cosmosiin (1), pedaliin (2), quercetin-3-O-rutinoside (3), pedaliin-6''-acetate (4), and sorbifolin-6-O-beta-glucopyranoside (5). The current strategy provides a reference for separating compounds with similar polarities from a crude sample.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61345
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, T,Yang, X,Wang, S,et al. Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes[J]. JOURNAL OF SEPARATION SCIENCE,2022,45(2):468.
APA	Chen, T.,Yang, X.,Wang, S.,Shen, C.,Li, HM.,...&Li, YL.(2022).Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes.JOURNAL OF SEPARATION SCIENCE,45(2),468.
MLA	Chen, T,et al."Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes".JOURNAL OF SEPARATION SCIENCE 45.2(2022):468.