Integrated strategy for the screening of cyclooxygenase-2 inhibitors from triterpenoid saponins in Clematis tangutica

NWIPB OpenIR

	Integrated strategy for the screening of cyclooxygenase-2 inhibitors from triterpenoid saponins in Clematis tangutica
	Wei, Yangfei; Chen, Tao; Wang, Shuo; Shen, Cheng; Song, Zhibo; Li, Aijing; Li, Hongmei; Li, Yulin
	2023
发表期刊	PHYTOCHEMICAL ANALYSIS
卷号	34 期号:6 页码:692
摘要	IntroductionScreening of novel cyclooxygenase-2 (COX-2) inhibitors from complex natural products is not an easy task. ObjectivesTo establish an efficient and feasible strategy for screening COX-2 inhibitors from triterpenoid saponins (TPSs) in Clematis tangutica. MethodsTaking TPSs in C. tangutica as example, an optimized macroporous resin (MR) method was established for the enrichment of TPSs. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) was performed to establish the phytochemical profiling of TPSs. Molecular docking was performed to predict the ligand-target interactions and discover the active substances. Chemometric techniques were performed to visualize the structure-effect relationships. High-speed countercurrent chromatography and preparative HPLC were performed to prepare the targets. In vitro activity experiment of COX-2 was performed to verify the virtual screening results. ResultsTPSs in C. tangutica were well enriched with the recovery rate of (80.22 & PLUSMN; 2.37)%. Thirty-four kinds of TPSs of oleanane type were deduced by HPLC-QTOFMS. Five TPSs of clematangoside C, clematangoside D, clematangoticoside J, hederoside H-1, and hederasaponin B showed stronger binding abilities with COX-2. The structure with more sugar groups at C-28 may be more conducive to the combination with COX-2. Targets were prepared with purities all above 98%. The IC50 values of target TPSs were 6.03 & PLUSMN; 0.24, 12.44 & PLUSMN; 0.15, 9.36 & PLUSMN; 0.19, 4.78 & PLUSMN; 0.13, and 2.59 & PLUSMN; 0.11 & mu;mol/L, respectively. ConclusionThe integrated strategy using MR, HPLC-QTOFMS, molecular docking, chemometrics, target preparation, and in vitro verification was feasible for rapidly screening COX-2 inhibitors from TPSs in C. tangutica.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61615
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wei, Yangfei,Chen, Tao,Wang, Shuo,et al. Integrated strategy for the screening of cyclooxygenase-2 inhibitors from triterpenoid saponins in Clematis tangutica[J]. PHYTOCHEMICAL ANALYSIS,2023,34(6):692.
APA	Wei, Yangfei.,Chen, Tao.,Wang, Shuo.,Shen, Cheng.,Song, Zhibo.,...&Li, Yulin.(2023).Integrated strategy for the screening of cyclooxygenase-2 inhibitors from triterpenoid saponins in Clematis tangutica.PHYTOCHEMICAL ANALYSIS,34(6),692.
MLA	Wei, Yangfei,et al."Integrated strategy for the screening of cyclooxygenase-2 inhibitors from triterpenoid saponins in Clematis tangutica".PHYTOCHEMICAL ANALYSIS 34.6(2023):692.