An Integrated Strategy for Investigating Antioxidants from Ribes himalense Royle ex Decne and Their Potential Target Proteins

NWIPB OpenIR

	An Integrated Strategy for Investigating Antioxidants from Ribes himalense Royle ex Decne and Their Potential Target Proteins
	Liu, Chuang; Lei, Yuqing; Liu, Youyi; Guo, Jingrou; Chen, Xingyi; Tang, Yifei; Dang, Jun; Wu, Minchen
	2023
发表期刊	ANTIOXIDANTS
卷号	12 期号:4
摘要	Natural products have been used extensively around the world for many years as therapeutic, prophylactic, and health-promotive agents. Ribes himalense Royle ex Decne, a plant used in traditional Tibetan medicine, has been demonstrated to have significant antioxidant and anti-inflammatory properties. However, the material basis of its medicinal effects has not been sufficiently explored. In this study, we established an integrated strategy by online HPLC-1,1-diphenyl-2-picrylhydrazyl, medium-pressure liquid chromatography, and HPLC to achieve online detection and separation of antioxidants in Ribes himalense extracts. Finally, four antioxidants with quercetin as the parent nucleus were obtained, namely, Quercetin-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnopyranoside, Quercetin-3-O-beta-D-xylopyranosyl(1-2)-beta-D-glucopyranoside, Quercetin-3-O-beta-D-glucopyranoside, and Quercetin-3-O-beta-D-galactoside. Until now, the four antioxidants in Ribes himalense have not been reported in other literatures. Meanwhile, the free-radical-scavenging ability of them was evaluated by DPPH assay, and potential antioxidant target proteins were explored using molecular docking. In conclusion, this research provides insights into the active compounds in Ribes himalense which will facilitate the advancement of deeper studies on it. Moreover, such an integrated chromatographic strategy could be a strong driver for more efficient and scientific use of other natural products in the food and pharmaceutical industries.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61664
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Chuang,Lei, Yuqing,Liu, Youyi,et al. An Integrated Strategy for Investigating Antioxidants from Ribes himalense Royle ex Decne and Their Potential Target Proteins[J]. ANTIOXIDANTS,2023,12(4).
APA	Liu, Chuang.,Lei, Yuqing.,Liu, Youyi.,Guo, Jingrou.,Chen, Xingyi.,...&Wu, Minchen.(2023).An Integrated Strategy for Investigating Antioxidants from Ribes himalense Royle ex Decne and Their Potential Target Proteins.ANTIOXIDANTS,12(4).
MLA	Liu, Chuang,et al."An Integrated Strategy for Investigating Antioxidants from Ribes himalense Royle ex Decne and Their Potential Target Proteins".ANTIOXIDANTS 12.4(2023).