Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection

NWIPB OpenIR

	Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection
	Wang, Lei; Xu, Jinqing; Wang, Handong; Chen, Tongrui; You, En; Bian, Haiyan; Chen, Wenjie; Zhang, Bo; Shen, Yuhu
	2023
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	14
摘要	IntroductionOat (Avena sativa L.) is an important cereal crop grown worldwide for grain and forage, owing to its high adaptability to diverse environments. However, the genetic and genomics research of oat is lagging behind that of other staple cereal crops. MethodsIn this study, a collection of 288 oat lines originating worldwide was evaluated using 2,213 single nucleotide polymorphism (SNP) markers obtained from an oat iSelect 6K-beadchip array to study its genetic diversity, population structure, and linkage disequilibrium (LD) as well as the genotype-phenotype association for hullessness and lemma color. ResultsThe average gene diversity and polymorphic information content (PIC) were 0.324 and 0.262, respectively. The first three principal components (PCs) accounted for 30.33% of the genetic variation, indicating that the population structure of this panel of oat lines was stronger than that reported in most previous studies. In addition, accessions could be classified into two subpopulations using a Bayesian clustering approach, and the clustering pattern of accessions was closely associated with their region of origin. Additionally, evaluation of LD decay using 2,143 mapped markers revealed that the intrachromosomal whole-genome LD decayed rapidly to a critical r(2) value of 0.156 for marker pairs separated by a genetic distance of 1.41 cM. Genome-wide association study (GWAS) detected six significant associations with the hullessness trait. Four of these six markers were located on the Mrg21 linkage group between 194.0 and 205.7 cM, while the other two significant markers mapped to Mrg05 and Mrg09. Three significant SNPs, showing strong association with lemma color, were located on linkage groups Mrg17, Mrg18, and Mrg20. DiscussionOur results discerned relevant patterns of genetic diversity, population structure, and LD among members of a worldwide collection of oat landraces and cultivars proposed to be 'typical' of the Qinghai-Tibetan Plateau. These results have important implications for further studies on association mapping and practical breeding in high-altitude oat.
收录类别	SCIE
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/61670
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Lei,Xu, Jinqing,Wang, Handong,et al. Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection[J]. FRONTIERS IN PLANT SCIENCE,2023,14.
APA	Wang, Lei.,Xu, Jinqing.,Wang, Handong.,Chen, Tongrui.,You, En.,...&Shen, Yuhu.(2023).Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection.FRONTIERS IN PLANT SCIENCE,14.
MLA	Wang, Lei,et al."Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection".FRONTIERS IN PLANT SCIENCE 14(2023).