Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae) | |
Tian, Z. Z.1,2; Zhang, F. Q.3; Cai, Z. Y.1; Chen, S. L.1 | |
2016 | |
发表期刊 | GENETICS AND MOLECULAR RESEARCH |
卷号 | 15期号:3 |
文章类型 | Article |
摘要 | Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the Illumina HiSeq 2000 platform. All microsatellite primers were tested on 48 P. cathayana individuals from four locations on the Qinghai-Tibet Plateau. The observed heterozygosity ranged from 0.000 to 1.000, and the null-allele frequency ranged from 0.000 to 0.904. These microsatellite markers may be a useful tool in genetic studies on P. cathayana and closely related species. |
关键词 | Populus Cathayana Microsatellite Est Qinghai-tibet Plateau |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.4238/gmr.15038406 |
关键词[WOS] | QINGHAI-TIBETAN PLATEAU ; DIVERSITY ; CHINA ; LOCI |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(31270270 ; International Scientific and Technological Cooperation Projects of Qinghai Province(2014-HZ-812) ; CAS "Light of West China" Program ; Youth Innovation Promotion Association CAS ; 31400322) |
WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
WOS记录号 | WOS:000384881300037 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6402 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Key Lab Crop Mol Breeding, Xining, Qinghai Provinc, Peoples R China |
推荐引用方式 GB/T 7714 | Tian, Z. Z.,Zhang, F. Q.,Cai, Z. Y.,et al. Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)[J]. GENETICS AND MOLECULAR RESEARCH,2016,15(3). |
APA | Tian, Z. Z.,Zhang, F. Q.,Cai, Z. Y.,&Chen, S. L..(2016).Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae).GENETICS AND MOLECULAR RESEARCH,15(3). |
MLA | Tian, Z. Z.,et al."Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)".GENETICS AND MOLECULAR RESEARCH 15.3(2016). |
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