Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design

doi:10.1039/c6tb01570k

NWIPB OpenIR

	Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design
	Yang, Jingchang 1,2; Rong, Huan 1,2; Shao, Ping 3; Tao, Yanduo 3; Dang, Jun 3; Wang, Peng 4; Ge, Yushu 1,2; Wu, Jiang 4; Liu, Dan 1,2
	2016
发表期刊	JOURNAL OF MATERIALS CHEMISTRY B
卷号	4 期号:36 页码:6065-6073
文章类型	Article
摘要	Recently, fluorescent peptide-based chemosensors have been reported and used to detect series of metal ions in both aqueous solutions and living cells. In order to explore and indicate the structural regulations of ''fluorophore-(His)(n)-Pro-Gly-(His)(n)-fluorophore (H2L peptide sensor)'', we predict the relationship between the number of histidine molecules and the coordination activity, as well as the preferred coordination site, through computational studies. L-n=3 was modeled as showing the highest activity with the lowest calculated DEtotal, and inner histidine residues bonding with the ''Pro-Gly'' core structure always provided essential coordination sites for zinc ions. A predicted structural regulation mechanism was studied and verified experimentally. Three Zn2+ sensors with the number of histidine molecules set as 2 (L-n=2), 3 (L-n=3) and 4 (L-n=4) were synthesized and tested. The L-n=3 sensor shows the highest fluorescence intensity by specific binding with Zn2+. The key coordination site was identified by mutating the histidine residues of the L-n=3 sensor. The inner histidine bonding with the ''Pro-Gly'' core structure was proved as the vital coordination site, and at least one extra histidine had to be provided to assist the coordination process according to the experimental results. Both computational and experimental studies reveal the same relationship between the structure and activity for H2L peptides in the fluorescent sensing of zinc ions. The results give fundamental information for the reasonable design and optimization of all similar classes of peptide sensors.
WOS标题词	Science & Technology ; Technology
DOI	10.1039/c6tb01570k
关键词[WOS]	FLUORESCENT CHEMOSENSOR ; METAL-IONS ; AQUEOUS-SOLUTIONS ; HG(II) IONS ; AMINO-ACID ; SENSORS ; COPPER ; CU
收录类别	SCI
语种	英语
项目资助者	Recruitment Program for Young Professionals(KJ2070000027) ; Major State Special Research Project of China(2016YFA0101200) ; Major State Basic Research Development Program of China (973 Program)(2015CB553701) ; National Natural Science Foundation of China(21201091) ; Fundamental Research Funds for the Central Universities(lzujbky-2013-59)
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000384233900011
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6436
专题	中国科学院西北高原生物研究所
作者单位	1.Univ Sci & Technol China, Inst Immunol, Collaborat Innovat Ctr Chem Life Sci, Hefei 230027, Peoples R China 2.Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, Sch Life Sci, Hefei 230027, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 4.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jingchang,Rong, Huan,Shao, Ping,et al. Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design[J]. JOURNAL OF MATERIALS CHEMISTRY B,2016,4(36):6065-6073.
APA	Yang, Jingchang.,Rong, Huan.,Shao, Ping.,Tao, Yanduo.,Dang, Jun.,...&Liu, Dan.(2016).Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design.JOURNAL OF MATERIALS CHEMISTRY B,4(36),6065-6073.
MLA	Yang, Jingchang,et al."Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design".JOURNAL OF MATERIALS CHEMISTRY B 4.36(2016):6065-6073.