Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| In situ situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry | |
| He, Yongrui1,2; Zhao, Xian-En1,2; Zhu, Shuyun1,2; Wei, Na1,2; Sun, Jing5,6; Zhou, Yubi5,6; Liu, Shu3,4; Liu, Zhiqiang3,4; Chen, Guang1,2; Suo, Yourui5,6; You, Jinmao1,2,5,6 | |
| 2016-08-05 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHY A
 |
| 卷号 | 1458页码:70-81 |
| 文章类型 | Article |
| 摘要 | Simultaneous monitoring of several neurotransmitters (NTs) linked to Parkinson's disease (PD) has important scientific significance for PD related pathology, pharmacology and drug screening. A new simple, fast and sensitive analytical method, based on in situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction (in situ DUADLLME) in a single step, has been proposed for the quantitative determination of catecholamines and their biosynthesis precursors and metabolites in rat brain microdialysates. The method involved the rapid injection of the mixture of low toxic bromobenzene (extractant) and acetonitrile (dispersant), which containing commercial Lissamine rhodamine B sulfonyl chloride (LRSC) as derivatization reagent, into the aqueous phase of sample and buffer, and the following in situ DUADLLME procedure. After centrifugation, 50 mu L of the sedimented phase (bromobenzene) was directly injected for ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) detection in multiple reaction monitoring (MRM) mode. This interesting combination brought the advantages of speediness, simpleness, low matrix effects and high sensitivity in an effective way. Parameters of in situ DUADLLME and UHPLC-MS/MS conditions were all optimized in detail. The optimum conditions of in situ DUADLLME were found to be 30 mu L of microdialysates, 150 mu L of acetonitrile containing LRSC, 50 mu L of bromobenzene and 800 mu L of NaHCO3-Na2CO3 buffer (pH 10.5) for 3.0 min at 37 degrees C. Under the optimized conditions, good linearity was observed with LODs (S/N > 3) and LOQs (S/N > 10) of LRSC derivatized-NTs in the range of 0.002-0.004 and 0.007-0.015 nmol/L, respectively. It also brought good precision (3.2-12.8%, peak area CVs%), accuracy (94.2-108.6%), recovery (94.5-105.5%) and stability (3.8-8.1%, peak area CVs%) results. Moreover, LRSC derivatization significantly improved chromatographic resolution and MS detection sensitivity of NTs when compared with the reported studies through the introduction of a permanent charged moiety from LRSC into NTs. Taken together, this in situ DUADLLME method was successfully applied for the simultaneous determination of six NTs in biological samples. (C) 2016 Elsevier B.V. All rights reserved. |
| 关键词 | In Situ Derivatization Ultrasound-assisted Dispersive Liquid-liquid Microextraction In Vivo Microdialysis Neurotransmitters Ultra High Performance Liquid Chromatography-tandem Mass Spectrometry Traditional Chinese Medicine |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.chroma.2016.06.059 |
| 关键词[WOS] | ENVIRONMENTAL WATER SAMPLES ; AMINO-ACID NEUROTRANSMITTERS ; SOLID-PHASE EXTRACTION ; MONOAMINE NEUROTRANSMITTERS ; ULTRAVIOLET FILTERS ; LC-MS/MS ; DOPAMINE ; CATECHOLAMINES ; METABOLITES ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China(81303179 ; Natural Science Foundation of Shandong Province(ZR20138Q018 ; Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Development Project of Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources(2014-Z-Y3) ; Project of Application Institute of New Technology of Alxa & Chinese Academy of Sciences ; Foundation of Qufu Normal University(BSQD2012019 ; 21405094 ; ZR20138Q019 ; 2012023) ; 21475074 ; ZR2015CM040) ; 21275089 ; 81573574 ; 81530094 ; 81403051) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000380972800009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/6441 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, Natl Ctr Mass Spectrometry Changchun, 5625 Renmin St, Changchun 130022, Peoples R China 4.Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Tradit Chinese Med Chem & Mass Spectromet, 5625 Renmin St, Changchun 130022, Peoples R China 5.Chinese Acad Sci, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 6.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Yongrui,Zhao, Xian-En,Zhu, Shuyun,et al. In situ situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1458:70-81. |
| APA | He, Yongrui.,Zhao, Xian-En.,Zhu, Shuyun.,Wei, Na.,Sun, Jing.,...&You, Jinmao.(2016).In situ situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1458,70-81. |
| MLA | He, Yongrui,et al."In situ situ derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1458(2016):70-81. |
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