Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography

doi:10.1093/chromsci/bmv249

NWIPB OpenIR

	Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography
	Lv, Huanhuan 1,2; Zhou, Wenna 1,2; Wang, Xiaoyan 1; Wang, Zhenhua 1,3; Suo, Yourui 1; Wang, Honglun 1
	2016-05-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	54 期号:5 页码:744-751
文章类型	Article
摘要	Naturally occurring oligostilbenes are receiving more attention because they exhibit several beneficial effects for health, including hepatoprotective, antitumor, anti-adipogenic, antioxidant, antiaging, anti-inflammatory, anti-microbial, antiviral, immunosuppressive and neuroprotective activities. Thus, they could be of some potentially therapeutic values for several diseases. In this study, we adopted the alkaline extraction-acid precipitation (AEAP) method for extraction of oligostilbenes from the seed kernel of Iris lactea. Then, the high-speed counter-current chromatography (HSCCC) was used for preparative isolation and purification of oligostilbenes from the AEAP extracts. Finally, three oligostilbenes, namely vitisin D (73 mg), ampelopsin B (25 mg) and cis-vitisin A (16 mg), were successfully fractionated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:5:3:6, v/v/v/v) from 300 mg of the AEAP extracts in similar to 190 min. The purities of the three isolated oligostilbenes were all over 95.0% as analyzed by high performance liquid chromatography. They all were isolated from I. lactea for the first time. The method of AEAP for the preparation of the oligostilbene-enriched crude sample was simple, and the HSCCC technique for the isolation and purification of oligostilbenes was efficient.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1093/chromsci/bmv249
关键词[WOS]	OLIGOSTILBENES ; AMURENSIS ; STEM ; LEAF ; STILBENES ; ANTIOXIDANT ; RESVERATROL ; CELLS ; MICE
收录类别	SCI
语种	英语
项目资助者	National Science Foundation of China(31470426 ; International Cooperation Project of Qinghai Province(2013-H-802) ; Qinghai Provincial Natural Science Foundation(2014-ZJ-765 ; Taishan Scholar Program of Shangdong Province(tshw201502046) ; Youth Innovation Promotion Association, CAS ; 31300292) ; 2015-ZJ-728)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000381702100009
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6442
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,et al. Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(5):744-751.
APA	Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,Wang, Zhenhua,Suo, Yourui,&Wang, Honglun.(2016).Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(5),744-751.
MLA	Lv, Huanhuan,et al."Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.5(2016):744-751.