Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography

doi:10.1080/01496395.2016.1191509

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	Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography
	Yuan, Zhenzhen 1,2,3; Lv, Huanhuan 1,3; Wang, Xiaoyan 1; Li, Gang 1; Suo, Yourui 1; Zhang, Zhang 4; Wang, Honglun 1,2
	2016
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
卷号	51 期号:12 页码:2020-2027
文章类型	Article
摘要	Four tannins were isolated and identified from Potentilla parvifolia using high-speed counter-current chromatography (HSCCC) in this study. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:11:1.2:11, v/v/v/v) was chosen and yielded 18.8 mg of procyanidin B3, 9.8 mg of procyanidin B6, 9.1 mg of procyanidin B7, and 6.9 mg of 2,3,4,6-tetra-O-galloyl-D-glucose, all at over 95% purity, from 120 mg of the crude sample powder. The identifications were performed with H-1 NMR and C-13 NMR. This is the first report showing the presence of procyanidin B7 and 2,3,4,6-tetra-O-galloyl-D-glucose in the genus Potentilla, and procyanidin B3 and procyanidin B6 were isolated from P. parvifolia for the first time.
关键词	Hsccc Potentilla Parvifolia Tannins Proanthocyanidins Preparative Isolation
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1080/01496395.2016.1191509
关键词[WOS]	IN-VITRO ; HYDROLYZABLE TANNINS ; TERMINALIA-CHEBULA ; GLUCOSIDES ; EXTRACTS ; FRUITS
收录类别	SCI
语种	英语
项目资助者	National Science Foundation of China(31470426 ; Qinghai Provincial Science Foundation(2015-SF-121 ; Key Program of "The Dawn of West China" Talent Foundation of CAS ; Youth Innovation Promotion Association, CAS ; 31300292) ; 2015-ZJ-728 ; 2014-Z-768)
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000382372100009
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6447
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Huzhou Plateau Biol Resource Ctr Innovat, Northwest Inst Plateau Biol, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.JALA Co Ltd, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Zhenzhen,Lv, Huanhuan,Wang, Xiaoyan,et al. Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2016,51(12):2020-2027.
APA	Yuan, Zhenzhen.,Lv, Huanhuan.,Wang, Xiaoyan.,Li, Gang.,Suo, Yourui.,...&Wang, Honglun.(2016).Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY,51(12),2020-2027.
MLA	Yuan, Zhenzhen,et al."Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY 51.12(2016):2020-2027.