The characteristics and functions of a miniature inverted-repeat transposable element TaMITE81 in the 5' UTR of TaCHS7BL from Triticum aestivum

doi:10.1007/s00438-016-1234-8

NWIPB OpenIR

	The characteristics and functions of a miniature inverted-repeat transposable element TaMITE81 in the 5' UTR of TaCHS7BL from Triticum aestivum
	Xi, Xinyuan 1,2; Li, Na 1,2; Li, Shiming 1,3; Chen, Wenjie 1,3; Zhang, Bo 1,3; Liu, Baolong 1,3; Zhang, Huaigang 1,3
	2016-10-01
发表期刊	MOLECULAR GENETICS AND GENOMICS
卷号	291 期号:5 页码:1991-1998
文章类型	Article
摘要	Miniature inverted-repeat transposable elements (MITEs) are truncated derivatives of autonomous DNA transposons, and are dispersed abundantly in eukaryotic and prokaryotic genomes. In this article, a MITE, TaMITE81, was isolated from the 5' untranslated region (UTR) of TaCHS7BL, chalcone synthase (CHS) catalyzing the first committed step of anthocyanin biosynthesis, in the wheat cultivar 'Opata' with white grain. TaMITE81 was only 81 nucleotides, including a terminal inverted repeat with 39 nucleotides and was flanked by two nucleotides, "TA", target site duplications that were typical features of stowaway-like MITEs. Compared with the wheat cultivar 'Gy115' with purple grain, which is without the insertion, the expression of TaCHS7BL was lower in several organs of 'Opata'. The insertion of TaMITE81 into the 5' UTR of the GUS gene also reduced the transient expression of GUS on the coleoptiles of 'Opata', which means the insertion of TaMITE81 was the reason for the low expression of TaCHS7BL in 'Opata'. But the genotype of TaCHS7BL was not linked to phenotype of grain color in the RILs derived from a cross 'Gy115' and 'Opata'. The TaMITE81 density of the hexaploid variety of T. aestivum was more than 10 times that of diploid relatives, which implies that polyploidization caused the amplification of TaMITE81 homologous sequences. Further research should be conducted on decoding the relationship between TaCHS7BL and other traits relative to anthocyanin biosynthesis in wheat, and discovering the mechanism of TaMITE81 transposon action.
关键词	Mite Chalcone Synthase Characteristics Function Genetic Diversity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00438-016-1234-8
关键词[WOS]	ANTHOCYANIN BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; ORYZA-SATIVA ; WHEAT ; EXPRESSION ; MITES ; GENOME ; GENES ; AMPLIFICATION ; FAMILY
收录类别	SCI
语种	英语
项目资助者	Pilot Projects of Designer Breeding by Molecular Module ; West Light Foundation of Chinese Academy of Sciences ; Basic Research Projects of Qinghai Province(2015-ZJ-701) ; National Natural Science Foundation of China(31071417)
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000382145100015
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6451
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Xi, Xinyuan,Li, Na,Li, Shiming,et al. The characteristics and functions of a miniature inverted-repeat transposable element TaMITE81 in the 5' UTR of TaCHS7BL from Triticum aestivum[J]. MOLECULAR GENETICS AND GENOMICS,2016,291(5):1991-1998.
APA	Xi, Xinyuan.,Li, Na.,Li, Shiming.,Chen, Wenjie.,Zhang, Bo.,...&Zhang, Huaigang.(2016).The characteristics and functions of a miniature inverted-repeat transposable element TaMITE81 in the 5' UTR of TaCHS7BL from Triticum aestivum.MOLECULAR GENETICS AND GENOMICS,291(5),1991-1998.
MLA	Xi, Xinyuan,et al."The characteristics and functions of a miniature inverted-repeat transposable element TaMITE81 in the 5' UTR of TaCHS7BL from Triticum aestivum".MOLECULAR GENETICS AND GENOMICS 291.5(2016):1991-1998.