Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent

doi:10.1039/c6ra11633g

NWIPB OpenIR

	Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent
	Qu, Fengli 1,2; Xia, Lian 1,2; Wu, Chuanxiang 1,2; Liu, Lijie 1,2; Li, Guoliang 1,2; You, Jinmao 1,2,3
	2016
发表期刊	RSC ADVANCES
卷号	6 期号:69 页码:64895-64901
文章类型	Article
摘要	A zirconium-based metal-organic frameworks (MOF), with 2-aminoterephthalic acid as linkers, named UiO-66-NH2, has been synthetized and characterized in a dispersive solid-phase extraction (DSPE) procedure in combination with high-performance liquid chromatography (HPLC) with fluorescence detection (FLD) for the pre-concentration and detection of sialic acids (SAs) in serum samples. In this study, UiO-66-NH2 was employed for the first time as a novel DSPE sorbent for sample pretreatment. Parameters which influence the extraction efficiency such as the amount of MOF, nature of the desorption solvent and its amount, adsorption time and desorption time were investigated and optimized. Under the optimized conditions, better extraction results were obtained by UiO-66-NH2 based DSPE for the extraction of SAs from serum samples than traditional sample preparation methods. Detection limits of the method as low as 0.16 pmol for N-glycolylneuraminic acid (Neu5Gc) and 0.11 pmol for N-acetylneuraminic acid (Neu5Ac) were obtained, with determination coefficients (R-2) higher than 0.9995 for the range 10-1000 pmol L-1, average recoveries of >91.3% at three different spiked levels (1, 5 and 10 ng mL(-1)), and relative standard deviation (RSD) values below 3.4%. The proposed method was demonstrated to be sensitive enough for determination of SAs from bio-samples.
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1039/c6ra11633g
关键词[WOS]	METAL-ORGANIC FRAMEWORK ; N-GLYCOLYLNEURAMINIC ACIDS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; LIGHT-DRIVEN PHOTOCATALYST ; ESI-MS ; AQUEOUS CR(VI) ; HUMAN CANCER ; SEPARATION ; DERIVATIZATION ; REDUCTION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21505084 ; Natural Science Foundation of Shandong Province(ZR2014BM029) ; 21475075 ; 21375076 ; 31301595 ; 21475074)
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000379442400082
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6470
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med S, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Qu, Fengli,Xia, Lian,Wu, Chuanxiang,et al. Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent[J]. RSC ADVANCES,2016,6(69):64895-64901.
APA	Qu, Fengli,Xia, Lian,Wu, Chuanxiang,Liu, Lijie,Li, Guoliang,&You, Jinmao.(2016).Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent.RSC ADVANCES,6(69),64895-64901.
MLA	Qu, Fengli,et al."Sensitive and accurate determination of sialic acids in serum with the aid of dispersive solid-phase extraction using the zirconium-based MOF of UiO-66-NH2 as sorbent".RSC ADVANCES 6.69(2016):64895-64901.