Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC

doi:10.1007/s10337-016-3090-4

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	Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC
	Luan, Guangxiang 1,3; Wang, Honglun 1,2; Lv, Huanhuan 1,3; Hu, Na 1; Suo, Yourui 1; Wang, Xiaoyan 1
	2016-07-01
发表期刊	CHROMATOGRAPHIA
卷号	79 期号:13-14 页码:823-831
文章类型	Article
摘要	A method of using high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) to preparatively separate flavone glucosides and lignan from the crude extracts of Caragana korshinskii has been established for the first time in this study. Five flavone glucosides, including 9 mg of kaempferol 3-O-{beta-d-glucopyranosyl(1 -> 2)-[alpha-l-rhamonopyranosyl(1 -> 6)]-beta-d-galactopyranoside}, 21 mg of kaempferol 3-O-alpha-l-rhamnopyranosyl(1 -> 6)-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 34 mg of kaempferol 3-O-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 27 mg of kaempferol 3-O-beta-d-glucopyranosyl-7-O-alpha-l-rhamnopyranoside, and 14 mg of calycosin 7-O-beta-d-glucopyranoside, and one lignan, 16 mg of alangilignoside B, were successfully isolated from 1.8 g of the crude sample through the combination of HSCCC with a two-phase solvent system composed of ethyl acetate-n-butanol-0.5 % formic acid (4:1:5, v/v/v) and semi-preparative RPLC with a mobile phase of methanol-water (20:80, v/v). The purities of the six compounds are all over 95 % as determined by HPLC and the structures are confirmed by the analysis of UV, H-1-NMR, and C-13-NMR and compared with published data.
关键词	Hsccc Semi-preparative Rplc Caragana Korshinskii Kom Flavone Glucosides Lignan Separation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1007/s10337-016-3090-4
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; GLYCOSIDES ; ISOFLAVONES ; CHINENSIS ; SHIFT ; SEEDS ; HPLC
收录类别	SCI
语种	英语
项目资助者	National Science Foundation of China(31470426 ; Qinghai Provincial Science Foundation(2015-SF-121 ; "The Dawn of West China" Talent Foundation of CAS ; Youth Innovation Promotion Association, CAS ; 31300292) ; 2015-ZJ-728 ; 2014-Z-768)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000379157500003
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6475
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Huzhou Plateau Biol Resource Ctr Innovat, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,et al. Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC[J]. CHROMATOGRAPHIA,2016,79(13-14):823-831.
APA	Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,Hu, Na,Suo, Yourui,&Wang, Xiaoyan.(2016).Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC.CHROMATOGRAPHIA,79(13-14),823-831.
MLA	Luan, Guangxiang,et al."Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC".CHROMATOGRAPHIA 79.13-14(2016):823-831.