Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography

doi:10.1021/acs.jafc.6b02096

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	Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography
	Wang, Yan-Ming 1,2,4; Zhao, Jian-Qiang 3,4; Yang, Jun-Li 1,2; Tao, Yan-Duo 3; Mei, Li-Juan 3; Shi, Yan-Ping 1,2
	2016-06-22
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷号	64 期号:24 页码:4950-4957
文章类型	Article
摘要	Asterothamnus centrali-asiaticus, a kind of characteristic shrub abundant in grassland and desert areas, has been used as forage fodder for camels and goats in Central Asia, and this plant also plays a critical role in the maintenance of desert grassland ecosystems as a result of its tolerance to poor soils and sand burial. However, its chemical composition has been rarely reported. In this study, phytochemical investigation of this pasturage was performed and three new triterpenoid saponins (1-3) were isolated together with nine known triterpenoid saponins (4-12) using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography (2D RPLC/HILIC). Their structures were elucidated via diverse spectroscopic analyses, including infrared (IR) spectrometry, high-resolution electrospray ionization mass spectrometry (HR-ESIMS), and one-dimensional (1D) and 2D nuclear magnetic resonance (NMR). All isolated triterpenoid saponins (1-12) were reported from this genus for the first time, and they were further evaluated for their cytotoxicity against four cancer cell lines (A549, HepG2, MGC-803, and MFC), which indicated that compound 11 showed potent cytotoxicity against the HepG2 cell line, with an IC50 value of 6.85 mu g/mL.
关键词	Asterothamnus Centrali-asiaticus Triterpenoid Saponins 2d Rplc/hilic Cytotoxicity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1021/acs.jafc.6b02096
关键词[WOS]	HYDROPHILIC INTERACTION CHROMATOGRAPHY ; TRITERPENOID SAPONINS ; PLATYCODON-GRANDIFLORUM ; ASTER-AURICULATUS ; PURIFICATION ; SEPARATION ; BUFADIENOLIDES ; GINSENOSIDES ; COLUMN ; ROOTS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21375136 ; scientific research project of Central Asia Drug Discovery and Development Centre of Chinese Academy of Sciences(CAM201404) ; CAS Pioneer Hundred Talents Program ; 21575150)
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:000378584300016
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6481
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,et al. Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(24):4950-4957.
APA	Wang, Yan-Ming,Zhao, Jian-Qiang,Yang, Jun-Li,Tao, Yan-Duo,Mei, Li-Juan,&Shi, Yan-Ping.(2016).Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(24),4950-4957.
MLA	Wang, Yan-Ming,et al."Isolation and Identification of Saponins from the Natural Pasturage Asterothamnus centrali-asiaticus Employing Preparative Two-Dimensional Reversed-Phase Liquid Chromatography/Hydrophilic Interaction Chromatography".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.24(2016):4950-4957.