Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat

doi:10.1556/0806.43.2015.030

NWIPB OpenIR

	Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat
	Wang, Y. Q.1,3,4; Hou, X. J.2; Zhang, B.1,3; Chen, W. J.1,3; Liu, D. C.1,3; Liu, B. L.1,3; Zhang, H. G.1,3
	2016-03-01
发表期刊	CEREAL RESEARCH COMMUNICATIONS
卷号	44 期号:1 页码:35-46
文章类型	Article
摘要	Red coleoptile is an easily observed agronomic trait of wheat and has been extensively studied. However, the molecular mechanism of this trait has not yet been revealed. In this study, the MYB gene TaMYB-D1 was isolated from the wheat cultivar 'Gy115', which possesses red coleoptiles. This gene resided at the short arm of the homoelogous group 7 chromosomes. TaMYB-D1 was the only gene expressed in the coleoptiles of 'Gy115' and was not expressed in 'Opata' and 'CS', which have uncoloured coleoptiles. Phylogenetic analysis placed TaMYB-D1 very close to ZmC1 and other MYB proteins regulating anthocyanin biosynthesis. The encoded protein of TaMYB-D1 had an integrated DNA binding domain of 102 amino acids and a transcription domain with 42 amino acids, similar to the structure of ZmC1. Transient expression analysis in onion epidermal cells showed that TaMYB-D1 was located at the plant nucleus, which suggested its role as a transcription factor. The expression of TaMYB-D1 was accompanied with the expression of TaDFR and anthocyanin biosynthesis in the development of the coleoptile of 'Gy115'. Transient expression analysis showed that only TaMYB-D1 induced a few 'Opata' coleoptile cells to synthesize anthocyanin in light, and the gene also induced a colour change to red in many cells with the help of ZmR. All of these results suggested TaMYB-D1 as the candidate gene for the red coleoptile trait of 'Gy115'.
关键词	Triticum Aestivum Red Coleoptile Anthocyanin Biosynthesis Regulatory Network
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1556/0806.43.2015.030
关键词[WOS]	ANTHOCYANIN BIOSYNTHESIS ; TRANSCRIPTION FACTOR ; FLAVONOID BIOSYNTHESIS ; DRAFT GENOME ; MAIZE C1 ; EXPRESSION ; SEQUENCE ; VVMYBA1 ; PIGMENTATION ; REGULATOR
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31071417 ; West Light Foundation of the Chinese Academy of Sciences ; Pilot Projects of Designer Breeding by Molecular Modules, Chinese Academy of Sciences ; 31260322)
WOS研究方向	Agriculture
WOS类目	Agronomy
WOS记录号	WOS:000378377300004
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6485
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Qinghai Xining 810008, Peoples R China 2.Qinghai Normal Univ, Coll Biol & Geog Sci, Qinghai Xining 810008, Peoples R China 3.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Y. Q.,Hou, X. J.,Zhang, B.,et al. Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat[J]. CEREAL RESEARCH COMMUNICATIONS,2016,44(1):35-46.
APA	Wang, Y. Q..,Hou, X. J..,Zhang, B..,Chen, W. J..,Liu, D. C..,...&Zhang, H. G..(2016).Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat.CEREAL RESEARCH COMMUNICATIONS,44(1),35-46.
MLA	Wang, Y. Q.,et al."Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat".CEREAL RESEARCH COMMUNICATIONS 44.1(2016):35-46.