Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat | |
Wang, Y. Q.1,3,4; Hou, X. J.2; Zhang, B.1,3; Chen, W. J.1,3; Liu, D. C.1,3; Liu, B. L.1,3; Zhang, H. G.1,3 | |
2016-03-01 | |
发表期刊 | CEREAL RESEARCH COMMUNICATIONS
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卷号 | 44期号:1页码:35-46 |
文章类型 | Article |
摘要 | Red coleoptile is an easily observed agronomic trait of wheat and has been extensively studied. However, the molecular mechanism of this trait has not yet been revealed. In this study, the MYB gene TaMYB-D1 was isolated from the wheat cultivar 'Gy115', which possesses red coleoptiles. This gene resided at the short arm of the homoelogous group 7 chromosomes. TaMYB-D1 was the only gene expressed in the coleoptiles of 'Gy115' and was not expressed in 'Opata' and 'CS', which have uncoloured coleoptiles. Phylogenetic analysis placed TaMYB-D1 very close to ZmC1 and other MYB proteins regulating anthocyanin biosynthesis. The encoded protein of TaMYB-D1 had an integrated DNA binding domain of 102 amino acids and a transcription domain with 42 amino acids, similar to the structure of ZmC1. Transient expression analysis in onion epidermal cells showed that TaMYB-D1 was located at the plant nucleus, which suggested its role as a transcription factor. The expression of TaMYB-D1 was accompanied with the expression of TaDFR and anthocyanin biosynthesis in the development of the coleoptile of 'Gy115'. Transient expression analysis showed that only TaMYB-D1 induced a few 'Opata' coleoptile cells to synthesize anthocyanin in light, and the gene also induced a colour change to red in many cells with the help of ZmR. All of these results suggested TaMYB-D1 as the candidate gene for the red coleoptile trait of 'Gy115'. |
关键词 | Triticum Aestivum Red Coleoptile Anthocyanin Biosynthesis Regulatory Network |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1556/0806.43.2015.030 |
关键词[WOS] | ANTHOCYANIN BIOSYNTHESIS ; TRANSCRIPTION FACTOR ; FLAVONOID BIOSYNTHESIS ; DRAFT GENOME ; MAIZE C1 ; EXPRESSION ; SEQUENCE ; VVMYBA1 ; PIGMENTATION ; REGULATOR |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(31071417 ; West Light Foundation of the Chinese Academy of Sciences ; Pilot Projects of Designer Breeding by Molecular Modules, Chinese Academy of Sciences ; 31260322) |
WOS研究方向 | Agriculture |
WOS类目 | Agronomy |
WOS记录号 | WOS:000378377300004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6485 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Qinghai Xining 810008, Peoples R China 2.Qinghai Normal Univ, Coll Biol & Geog Sci, Qinghai Xining 810008, Peoples R China 3.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Y. Q.,Hou, X. J.,Zhang, B.,et al. Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat[J]. CEREAL RESEARCH COMMUNICATIONS,2016,44(1):35-46. |
APA | Wang, Y. Q..,Hou, X. J..,Zhang, B..,Chen, W. J..,Liu, D. C..,...&Zhang, H. G..(2016).Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat.CEREAL RESEARCH COMMUNICATIONS,44(1),35-46. |
MLA | Wang, Y. Q.,et al."Identification of a Candidate Gene for Rc-D1, a Locus Controlling Red Coleoptile Colour in Wheat".CEREAL RESEARCH COMMUNICATIONS 44.1(2016):35-46. |
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