PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa

doi:10.1038/srep18643

NWIPB OpenIR

	PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa
	Yang, Li 1; Zhao, Xin 1; Yang, Fan 1; Fan, Di 1; Jiang, Yuanzhong 1; Luo, Keming 1,2
	2016-01-28
发表期刊	SCIENTIFIC REPORTS
卷号	6
文章类型	Article
摘要	WRKY proteins are one of the largest transcription factor families in higher plants and play diverse roles in various biological processes. Previous studies have shown that some WRKY members act as negative regulators of secondary cell wall formation in pith parenchyma cells. However, the regulatory mechanism of pith secondary wall formation in tree species remains largely unknown. In this study, PtrWRKY19 encoding a homolog of Arabidopsis WRKY12 was isolated from Populus trichocarpa. PtrWRKY19 was expressed in all tissues tested, with highest expression in stems, especially in pith. PtrWRKY19 was located in the nucleus and functioned as a transcriptional repressor. Ectopic expression of PtrWRKY19 in an atwrky12 mutant successfully rescued the phenotype in pith cell walls caused by the defect of AtWRKY12, suggesting that PtrWRKY19 had conserved functions for homologous AtWRKY12. Overexpression of PtrWRKY19 in poplar plants led to a significant increase in the number of pith parenchyma cells. qRT-PCR analysis showed that lignin biosynthesis-related genes were repressed in transgenic plants. In transcient reporter assays, PtrWRKY19 was identified to repress transcription from the PtoC4H2 promoter containing the conserved W-box elements. These results indicated that PtrWRKY19 may function as a negative regulator of pith secondary wall formation in poplar.
WOS标题词	Science & Technology
DOI	10.1038/srep18643
关键词[WOS]	ARABIDOPSIS-THALIANA ; ANTHER DEHISCENCE ; ABIOTIC STRESSES ; WOOD FORMATION ; BIOSYNTHESIS ; PLANTS ; GENE ; TRANSFORMATION ; POPLAR ; MYB
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31300990 ; Natural Science Foundation Project of CQ(CSTC2013JJB8007) ; Fundamental Research Funds for the Central Universities(XDJK2014a005) ; "One Hundred Talents Program" of the Chinese Academy of Sciences ; 31370672 ; 31171620)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000368781200001
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6495
专题	中国科学院西北高原生物研究所
作者单位	1.Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Yang, Li,Zhao, Xin,Yang, Fan,et al. PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa[J]. SCIENTIFIC REPORTS,2016,6.
APA	Yang, Li,Zhao, Xin,Yang, Fan,Fan, Di,Jiang, Yuanzhong,&Luo, Keming.(2016).PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa.SCIENTIFIC REPORTS,6.
MLA	Yang, Li,et al."PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa".SCIENTIFIC REPORTS 6(2016).