A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography

doi:10.1039/c5ay02648b

NWIPB OpenIR

	A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography
	Wu, Chuanxiang 1; Xia, Lian 1; Liu, Lijie 1; Qu, Fengli 1; Li, Guoliang 1; Sun, Zhiwei 1; Ji, Zhongyin 1; Sun, Weiwei 1; You, Jinmao 1,2
	2016
发表期刊	ANALYTICAL METHODS
卷号	8 期号:3 页码:554-563
文章类型	Article
摘要	A novel, sensitive and convenient method, utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization (UCSHD) prior to high performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD) and online mass spectrometry (MS) identification, has been developed for determination of sialic acids. The pivotal parameters affecting the release of sialic acids from serum and the derivatization were investigated using a response surface methodology (RSM). Under optimized conditions, the maximum yield of sialic acids release were achieved and they were labeled successfully in a relatively short time of 72 min (traditional time > 3 h) for the reason of the combination of hydrolysis steps with derivatization in a closed system with the assistance of ultrasonication. Excellent linearity (R-2 > 0.9991) in the calibration range of 0.5-16 mu mol mL(-1) and quite low detection limits (LODs) (0.30 pmol for Neu5Ac and 0.21 pmol for Neu5Gc) were achieved. When the established UCSHD-HPLC-FLD-MS method was applied to the analysis of sialic acids in various human sera, low relative error (RE: -3.4% to 2.5%), high recoveries (90-96%) and intra-and inter-day precisions (RSD, 0.9-2.2% for Neu5Ac and 1.4-2.8% for Neu5Gc) were also obtained, demonstrating obvious advantages for the accurate, sensitive and convenient determination of sialic acids in bio-samples.
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
DOI	10.1039/c5ay02648b
关键词[WOS]	N-GLYCOLYLNEURAMINIC ACIDS ; SOLID-PHASE EXTRACTION ; NEURAMINIC ACID ; CANCER-PATIENTS ; ACETYL ; FLUORESCENCE ; SEPARATION ; DISEASE ; SAMPLES ; URINE
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21505084 ; Natural Science Foundation of Shandong Province(ZR2014BM029 ; 21275089 ; ZR2012BQ022) ; 21475075 ; 21375076 ; 31301595 ; 21305076)
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS记录号	WOS:000367985300011
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6502
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Wu, Chuanxiang,Xia, Lian,Liu, Lijie,et al. A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography[J]. ANALYTICAL METHODS,2016,8(3):554-563.
APA	Wu, Chuanxiang.,Xia, Lian.,Liu, Lijie.,Qu, Fengli.,Li, Guoliang.,...&You, Jinmao.(2016).A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography.ANALYTICAL METHODS,8(3),554-563.
MLA	Wu, Chuanxiang,et al."A novel, sensitive and convenient method for determination of sialic acids in human serum utilizing ultrasonic-assisted closed in-syringe hydrolysis and derivatization prior to high performance liquid chromatography".ANALYTICAL METHODS 8.3(2016):554-563.