Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography | |
| Zhang, Qiulong1,2; Hu, Na1,2; Li, Wencong1,2; Ding, Chenxi3; Ma, Tao1,2; Bai, Bo1; Wang, Honglun1; Suo, Yourui1; Wang, Xiaoyan1; Ding, Chenxu1,4,5 | |
| 2015-09-01 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 卷号 | 53期号:8页码:1341-1345 |
| 文章类型 | Article |
| 摘要 | High-speed counter-current chromatography (HSCCC) was successfully applied for the preparative separation and purification of N-feruloyl serotonin (NF) and N-(p-coumaroyl) serotonin (NP) from safflower seed meal. After the measurement of partition coefficient of the two target compounds in the two-phase solvent systems, the HSCCC was performed well with a two-phase solvent system composed of CHCl3-methanol-0.1 M HCl at a volume ratio of 1 : 1 : 1, v/v. The upper phase was used as stationary phase and the lower phase was used as mobile phase. Under the optimized condition, 7.5 mg NF and 6.9 mg NP were separated from 40 mg crude sample with the purity of 98.8 and 97.3%, respectively. The structures of the isolated compounds were identified by H-1 NMR and C-13 NMR. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1093/chromsci/bmv018 |
| 关键词[WOS] | CARTHAMUS-TINCTORIUS L ; CONJUGATED SEROTONINS ; DERIVATIVES ; INHIBITION ; PLANT |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Chinese Ministry of Education(NO212188) ; Bureau of Science and Technology(2014-H-810) ; State Key Laboratory Breeding Base (Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000366629000016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/6510 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Red Cross Hosp, Xining 810099, Peoples R China 4.Qinghai Nationalities Univ, Xining 810007, Peoples R China 5.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qiulong,Hu, Na,Li, Wencong,et al. Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(8):1341-1345. |
| APA | Zhang, Qiulong.,Hu, Na.,Li, Wencong.,Ding, Chenxi.,Ma, Tao.,...&Ding, Chenxu.(2015).Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(8),1341-1345. |
| MLA | Zhang, Qiulong,et al."Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.8(2015):1341-1345. |
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