Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy

doi:10.1093/chromsci/bmv049

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	Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy
	Song, Cuihua 1; Zhang, Shijuan 1; Ji, Zhongyin 1,2; Li, Yipeng 3; You, Jinmao 1,2
	2015-10-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	53 期号:9 页码:1536-1541
文章类型	Article
摘要	An accurate and sensitive liquid chromatography-tandem mass spectrometry method was developed for the analysis of amino acids (isoleucine, leucine, valine, tyrosine, phenylalanine and tryptophan) in serum samples using a stable isotope labeling strategy. Amino acid samples and standards were, respectively, derivatized by 10-methyl-acridone-2-sulfonyl chloride (d(0)-MASC) and its deuterated counterpart d(3)-MASC to form isotopic pairs which co-eluted and were detected by an MS detector at the same time. Accurate internal standard-based quantification was thereby achieved without the use of any internal standard analogy. The labeling reaction of MASC with amino acids is fast, simple and robust. Besides, derivatization increased the molecular weight of amino acids, and therefore they were shifted out of the background noise which was often observed in low mass region. The instrument LODs were in the range of 1.0-2.5 nmol/L. Linearities calculated by comparing theoretical peak area ratios of d(0)-/d(3)-MASC derivatives with the experimental peak area ratios were excellent with correlation coefficients of >0.995. The proposed method was successfully applied to the analysis of amino acids in serum samples with high sensitivity and accuracy.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1093/chromsci/bmv049
关键词[WOS]	CHRONIC KIDNEY-DISEASE ; PRECOLUMN DERIVATIZATION ; RELATIVE QUANTIFICATION ; BIOLOGICAL SAMPLES ; REAGENT ; METABOLITES
收录类别	SCI
语种	英语
项目资助者	National Science Foundation of China(21275089) ; Natural Science Foundation of Shandong Province(ZR2014BQ009)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000366629600015
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6513
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 3.Qufu Peoples Hosp, Qufu, Peoples R China
推荐引用方式 GB/T 7714	Song, Cuihua,Zhang, Shijuan,Ji, Zhongyin,et al. Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(9):1536-1541.
APA	Song, Cuihua,Zhang, Shijuan,Ji, Zhongyin,Li, Yipeng,&You, Jinmao.(2015).Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(9),1536-1541.
MLA	Song, Cuihua,et al."Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.9(2015):1536-1541.

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