De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients

doi:10.1016/j.gene.2015.08.055

NWIPB OpenIR

	De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients
	Zhou, Dangwei 1,4; Gao, Shan 2; Wang, Huan 3; Lei, Tianxiang 1,4; Shen, Jianwei 3; Gao, Jie 1; Chen, Shilong1 ; Yin, Jia 1,4; Liu, Jianquan 1
	2016
发表期刊	GENE
卷号	575 期号:1 页码:160-170
文章类型	Article
摘要	Gentiana straminea is a popular Tibetan medicine that has been used for thousands of years in China to treat various diseases and conditions. Although it has multiple pharmaceutical purposes and important economic plant resource in China, transcriptome and molecular base still known limited. In flowering season, samples were collected from different tissues, using the NGS Illumina. Solexa platform, about 58.85 million sequencing reads were generated and assembled de novo, yielding 78,764 high quality unigenes with an average length of 1090 bp. Gene Ontology (GO), KEGG pathway mapping showed that 49,033 of these were identified as putative homologs of annotated sequences in the protein databases. Among them, candidate genes associated with iridoid, flavonoid and anthocyanin were identified. Further the key enzymes involved to iridoid and flavonoid synthesis pathway were analyzed by quantitative real-time polymerase chain reaction (gRT-PCR) on different tissues, the flower and root had the higher expression than leaves. In addition, 7591 SSR markers were identified from the unigenes of the G. straminea transcriptome. The foundation of G. straminea provided the important resource for facilitating to study molecular and functional genomics of it and related this species on the Qinghai-Tibet Plateau. (C) 2015 Elsevier B.V. All rights reserved.
关键词	G. Straminea Illumina Sequencing Transcriptome Flavonoids Iridoid Qrt-pcr Ssr Qinghai-tibet Plateau
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.gene.2015.08.055
关键词[WOS]	QINGHAI-TIBETAN PLATEAU ; OLEANOLIC ACID ; RNA-SEQ ; FUNCTIONAL GENOMICS ; URSOLIC ACID ; MARKERS ; CONSTITUENTS ; ARABIDOPSIS ; ANTIOXIDANT ; MACROPHYLLA
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31070208) ; Training Qualified People Plan "Hope of West China"(Y229151211 ; Key Innovation Plan of the Chinese Academy of Sciences(KSCX2-EW-J-26) ; Y129331211)
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000366226900020
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6519
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Dangwei,Gao, Shan,Wang, Huan,et al. De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients[J]. GENE,2016,575(1):160-170.
APA	Zhou, Dangwei.,Gao, Shan.,Wang, Huan.,Lei, Tianxiang.,Shen, Jianwei.,...&Liu, Jianquan.(2016).De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients.GENE,575(1),160-170.
MLA	Zhou, Dangwei,et al."De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients".GENE 575.1(2016):160-170.