Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Comparative transcriptome analysis of aboveground and underground tissues of Rhodiola algida, an important ethno-medicinal herb endemic to the Qinghai-Tibetan Plateau | |
Zhang, Faqi1,2; Gao, Qingbo1; Khan, Gulzar1; Luo, Keming1,2; Chen, Shilong1 ; Chen, SL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China.
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| 2014-12-15 | |
| 发表期刊 | GENE
 |
| 卷号 | 553期号:2页码:90-97 |
| 文章类型 | Article |
| 摘要 | Transcriptome sequencing is a powerful tool for the assessment of gene expression and the identification and characterization of molecular markers in non-model organisms. Rhodiola algida L. (Crassulaceae), endemic to the Qinghai-Tibetan Plateau, has long been used in traditional Chinese medicine to prevent altitude sickness and eliminate fatigue. Illumina-based high-throughput transcriptome sequencing of aboveground and underground tissues of R. algida respectively yielded 5.40 million and 5.18 million clean reads. A total of 82,664 unigenes averaging 577 bp in length were generated from the aboveground clean reads, with 86,237 unigenes of 502-bp mean length obtained from the underground tissues. Of 55,028 unigenes compared with sequences in UniProt databases, 20,413 unigenes had significant similarities with existing sequences in NR, NT, SwissProt, GO, KEGG, and COG databases. Single nucleotide polymorphism (SNP) analysis identified 237,294 SNPs from 154,636 contigs of aboveground tissues and 197,540 SNPs from 144,963 underground-derived contigs. The information uncovered in this study should serve as a valuable resource for the characterization of important traits related to secondary metabolite formation and for the identification of associated molecular mechanisms. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Rna-seq Transcriptome Rhodiola Algida Gene Expression |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | RNA-SEQ ; REFERENCE GENOME ; SNP DISCOVERY ; GENE ; ANNOTATION ; ALIGNMENT ; SEQUENCE ; PEPPER ; ATLAS ; PLANT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000345488400004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/6580 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Chen, SL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Faqi,Gao, Qingbo,Khan, Gulzar,et al. Comparative transcriptome analysis of aboveground and underground tissues of Rhodiola algida, an important ethno-medicinal herb endemic to the Qinghai-Tibetan Plateau[J]. GENE,2014,553(2):90-97. |
| APA | Zhang, Faqi,Gao, Qingbo,Khan, Gulzar,Luo, Keming,Chen, Shilong,&Chen, SL .(2014).Comparative transcriptome analysis of aboveground and underground tissues of Rhodiola algida, an important ethno-medicinal herb endemic to the Qinghai-Tibetan Plateau.GENE,553(2),90-97. |
| MLA | Zhang, Faqi,et al."Comparative transcriptome analysis of aboveground and underground tissues of Rhodiola algida, an important ethno-medicinal herb endemic to the Qinghai-Tibetan Plateau".GENE 553.2(2014):90-97. |
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| Comparative transcri（1043KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
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