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A New HMW-GS 1Bx23* Containing an Amino Acid Segment Similar to Collagen
Wei, L.1,2,3; Bai, S. G.4; Hou, X. J.2; Li, J. M.2; Zhang, B.1,3; Chen, W. J.1,3; Liu, D. C.1,3; Liu, B. L.1,3,5; Zhang, H. G.1,3; Liu, BL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810001, Qinghai, Peoples R China.
2014-12-01
发表期刊CEREAL RESEARCH COMMUNICATIONS
卷号42期号:4页码:658-667
文章类型Article
摘要Among 20 awnless Tibetan wheat cultivars analyzed by SDS-PAGE, the migration rate of an HMW-GS in XM001584 and XM001593, named 1Bx23*. was shown to be slightly faster than 1Bx6. and slower than Bx7. Its nucleotide sequence was isolated based on homology clones. In a phylogenetic tree of 1Bx genes, 1Bx23* was apparently clustered with 1Bx23. Compared with 1Bx23. eight single nucleotide replacements caused four single amino acid replacements in 1Bx23*. The deletion of "G" at base pair 1463 and insertion of "A" at 1509 bps induced a 42-nucleotide frame shift. "GQRQQAGQWQRPGQ" was replaced by "DKGNRQDNGNDRDK". The new segment cannot be found in other HMW-GSs, and it is very similar to a segment found in collagen. Moreover, an 18-nucleotide deletion made 1Bx23* six amino acids shorter than 1Bx23. The cultivar XM001593 had 28 chromosomes, which signifies that it was tetraploid wheat, and that the new HMW-GS1Bx23* cannot be used directly for breeding in common wheat.
关键词Tibetan Wheat Cultivar Hmw-gs Glu-bx Allelic Variation
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1556/CRC.2014.0012
关键词[WOS]WHEAT GLUTEN PROTEINS ; SUBUNITS ; GENETICS ; ELASTICITY ; EXPRESSION ; CLONING ; DNA
收录类别SCI
语种英语
WOS研究方向Agriculture
WOS类目Agronomy
WOS记录号WOS:000346507800012
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6581
专题中国科学院西北高原生物研究所
通讯作者Liu, BL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810001, Qinghai, Peoples R China.
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810001, Qinghai, Peoples R China
2.Qinghai Normal Univ, Coll Biol & Geog Sci, Xining 810008, Qinghai, Peoples R China
3.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810001, Peoples R China
4.Grassland Stn Huangzhong Cty, Xining 811600, Peoples R China
5.State Key Lab Breeding Base Innovat & Utilizat Pl, Xining 810001, Peoples R China
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GB/T 7714
Wei, L.,Bai, S. G.,Hou, X. J.,et al. A New HMW-GS 1Bx23* Containing an Amino Acid Segment Similar to Collagen[J]. CEREAL RESEARCH COMMUNICATIONS,2014,42(4):658-667.
APA Wei, L..,Bai, S. G..,Hou, X. J..,Li, J. M..,Zhang, B..,...&Liu, BL .(2014).A New HMW-GS 1Bx23* Containing an Amino Acid Segment Similar to Collagen.CEREAL RESEARCH COMMUNICATIONS,42(4),658-667.
MLA Wei, L.,et al."A New HMW-GS 1Bx23* Containing an Amino Acid Segment Similar to Collagen".CEREAL RESEARCH COMMUNICATIONS 42.4(2014):658-667.
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