Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum | |
| Chen, Tao1; Liu, Yong-Ling1,2; Chen, Chen1; Zou, Deng-Lang1,2; You, Jin-Mao1; Sun, Jing1; Li, Yu-Lin1; Sun, J (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. | |
| 2014-04-15 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 卷号 | 957页码:90-95 |
| 文章类型 | Article |
| 摘要 | In this paper, an efficient method was successfully established by the combination of macroporous resin (MR) and high-speed counter-current chromatography (HSCCC) for rapid enrichment and separation of aloe-emodin 8-O-beta-D-glucoside, emodin 1-O-beta-D-glucoside, emodin 8-O-beta-D-glucoside and piceatannol 4'-O-beta-D-(6 ''-O-gallate)-glucoside. Six kinds of macroporous resins were investigated in the first step and X-5 macroporous resin was selected for the enrichment of the target compounds. The recoveries of the target compounds reached 89.0, 85.9, 82.3 and 84.9% respectively after 40% ethanol elution. In the second step, the target compounds were separated by HSCCC with a two-phase solvent system composed of chloroform/ethyl acetate/methanol/water (8:1:6:5, v/v). The established method will be helpful for further characterization and utilization of Rheum tanguticum. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of bioactive compounds from natural products. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Rheum Tanguticum Anthraquinone Glycoside Stilbene Glycoside Macroporous Resin High-speed Counter-current Chromatography |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.jchromb.2014.02.045 |
| 关键词[WOS] | MAXIM. EX BALF. ; CHEMICAL-CONSTITUENTS ; PARTHENOCISSUS-TRICUSPIDATA ; POLYGONUM-MULTIFLORUM ; PREPARATIVE ISOLATION ; PURIFICATION ; RHUBARB ; IDENTIFICATION ; REQUIREMENTS ; DERIVATIVES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000350599300014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/6585 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Sun, J (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Liu, Yong-Ling,Chen, Chen,et al. Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,957:90-95. |
| APA | Chen, Tao.,Liu, Yong-Ling.,Chen, Chen.,Zou, Deng-Lang.,You, Jin-Mao.,...&Sun, J .(2014).Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,957,90-95. |
| MLA | Chen, Tao,et al."Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 957(2014):90-95. |
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