Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants

doi:10.1093/treephys/tpu079

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	Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants
	Ye, Shenglong 1; Jiang, Yuanzhong 1; Duan, Yanjiao 1; Karim, Abdul 1; Fan, Di 1; Yang, Li 1; Zhao, Xin 1; Yin, Jia 2; Luo, Keming 1,2; Luo, KM (reprint author), Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, 2 Tiansheng Rd, Chongqing 400715, Peoples R China.
	2014-10-01
发表期刊	TREE PHYSIOLOGY
卷号	34 期号:10 页码:1118-1129
文章类型	Article
摘要	WRKY proteins are involved in various physiological processes in plants, especially in coping with diverse biotic and abiotic stresses. However, limited information is available on the roles of specific WRKY transcription factors in poplar defense. In this study, we reported the characterization of PtoWRKY60, a Group IIa WRKY member, from Populus tomentosa Carr. The gene expression profile of PtoWRKY60 in various tissues showed that it significantly accumulated in old leaves. Phylogenetic analyses revealed that PtoWRKY60 had a close relationship with AtWRKY18, AtWRKY40 and AtWRKY60. PtoWRKY60 was induced mainly by salicylic acid (SA) and slightly by Dothiorella gregaria Sacc., jasmonic acid, wounding treatment, low temperature and salinity stresses. Overexpression of PtoWRKY60 in poplar resulted in increased resistance to D. gregaria. The defense-associated genes, such as PR5.1, PR5.2, PR5.4, PR5.5 and CPR5, were markedly up-regulated in transgenic plants overexpressing PtoWRKY60. These results indicate that PtoWRKY60 might be partly involved in the signal transduction pathway initiated by SA in Populus.
关键词	Pathogen Populus Salicylic Acid
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1093/treephys/tpu079
关键词[WOS]	SYSTEMIC ACQUIRED-RESISTANCE ; DNA-BINDING PROTEINS ; SALICYLIC-ACID ; ARABIDOPSIS-THALIANA ; DEFENSE RESPONSES ; PSEUDOMONAS-SYRINGAE ; SIGNALING PATHWAYS ; DISEASE-RESISTANCE ; PATHOGEN-DEFENSE ; GENE-EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000345838500009
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6588
专题	中国科学院西北高原生物研究所
通讯作者	Luo, KM (reprint author), Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, 2 Tiansheng Rd, Chongqing 400715, Peoples R China.
作者单位	1.Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Ye, Shenglong,Jiang, Yuanzhong,Duan, Yanjiao,et al. Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants[J]. TREE PHYSIOLOGY,2014,34(10):1118-1129.
APA	Ye, Shenglong.,Jiang, Yuanzhong.,Duan, Yanjiao.,Karim, Abdul.,Fan, Di.,...&Luo, KM .(2014).Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants.TREE PHYSIOLOGY,34(10),1118-1129.
MLA	Ye, Shenglong,et al."Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants".TREE PHYSIOLOGY 34.10(2014):1118-1129.

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