Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization | |
| Lv, Tao1,2; Zhao, Xian-En1,2; Zhu, Shuyun1,2; Ji, Zhongyin1,3; Chen, Guang1,2; Sun, Zhiwei1,2; Song, Cuihua1,2; You, Jinmao1,2,3; Suo, Yourui3; Zhao, XE (reprint author), Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China. | |
| 2014-12-01 | |
| 发表期刊 | CHROMATOGRAPHIA
 |
| 卷号 | 77期号:23-24页码:1653-1660 |
| 文章类型 | Article |
| 摘要 | A novel hyphenated technique based on ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) coupled with derivatization has been established for the determination of brassinolide (BL, a representative of brassinosteroids) by HPLC fluorescence detection. 9-Phenanthreneboronic acid is used as labeling reagent of BL. UA-DLLME parameters containing type and volume of extraction and disperser solvent, pH and ultrasonication time are optimized. Derivatization parameters are optimized included amount of 9-phenanthreneboronic acid, volume ratio of pyridine, derivatization time and temperature. Under optimal conditions, quantitative linear range of BL is 50-1,000 ng L-1 and excellent linear response is observed with correlation coefficient of 0.9996. Limit of detection and limit of quantification are calculated as 8.0 and 25.0 ng L-1, respectively. RSDs of retention time and peak area are in the range of 0.68-0.97 % and 4.61-6.54 % for intra-day precision, 1.32-1.94 % and 7.28-9.75 % for inter-day precision, respectively. Accuracy is satisfactory in the range of 82.3-125.1 %. RSDs' values of repeatability are in the range of 0.82-1.79 and 3.95-8.53 % for retention time and peak area, respectively. Enrichment factor for BL is 189. The results of recovery and matrix effect are in the range of 82.0-108.6 and 90.0-115.3 %, respectively. The proposed method has been applied for the determination of BL in Arabidopsis thaliana, Daucus carota and Brassica campestris L. leaves with much higher sensitivity than many other methods. |
| 关键词 | Column Liquid Chromatography Ultrasonic-assisted Dispersive Liquid-liquid Microextraction Derivatization Fluorescence Detection Brassinolide Plant Matrix |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1007/s10337-014-2767-9 |
| 关键词[WOS] | POLYMER MONOLITH MICROEXTRACTION ; CHROMATOGRAPHY-MASS-SPECTROMETRY ; SENSITIVE ANALYSIS ; BRASSINOSTEROIDS ; POLLEN ; PLANTS ; KUNTZE ; WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000345382600010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/6589 |
| 专题 | 中国科学院西北高原生物研究所 |
| 通讯作者 | Zhao, XE (reprint author), Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China. |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Tao,Zhao, Xian-En,Zhu, Shuyun,et al. Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization[J]. CHROMATOGRAPHIA,2014,77(23-24):1653-1660. |
| APA | Lv, Tao.,Zhao, Xian-En.,Zhu, Shuyun.,Ji, Zhongyin.,Chen, Guang.,...&Zhao, XE .(2014).Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization.CHROMATOGRAPHIA,77(23-24),1653-1660. |
| MLA | Lv, Tao,et al."Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization".CHROMATOGRAPHIA 77.23-24(2014):1653-1660. |
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