An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography

doi:10.1093/chromsci/bmw054

NWIPB OpenIR

	An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography
	Zou, Denglang 1,2,3; Chen, Tao 1,2; Chen, Chen 1,2; Li, Hongmei 1,2,3; Liu, Yongling 1,2; Li, Yulin 1,2
	2016-08-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	54 期号:7 页码:1220-1224
文章类型	Article
摘要	In this article, macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for preparation of gallic acid from Terminalia bellirica (Gaertn.) Roxb. In the first step, six kinds of resins were investigated by adsorption and desorption tests and AB-8 macroporous resin was selected for the enrichment of gallic acid. As a result, 20 g of gallic acid at a purity of 71% could be separated from 100 g of crude extract in which the content of gallic acid was 16.7% and the recovery of gallic acid reached 85.0%. In the second step, preparative high-performance liquid chromatography was selected to purify gallic acid. As a result, 640 mg of gallic acid at a purity of 99.1% was obtained from 1 g of sample in 35 min. The results demonstrated that macroporous resin coupled with preparative high-performance liquid chromatography was suitable for preparation of gallic acid from T. bellirica (Gaertn.) Roxb.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1093/chromsci/bmw054
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; ADSORPTION CHROMATOGRAPHY ; PURIFICATION ; SEPARATION ; ANTIOXIDANT ; BELERICA ; FRUITS ; EXTRACTION ; FLAVONOIDS ; ENRICHMENT
收录类别	SCI
语种	英语
项目资助者	General Program of Natural Science Foundation of Qinghai Province(2015-ZJ-908) ; Science and Technology Plan of Qinghai Province(2014-GX-C01 ; 2014-NS-515-2)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000389138700021
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6655
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zou, Denglang,Chen, Tao,Chen, Chen,et al. An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(7):1220-1224.
APA	Zou, Denglang,Chen, Tao,Chen, Chen,Li, Hongmei,Liu, Yongling,&Li, Yulin.(2016).An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(7),1220-1224.
MLA	Zou, Denglang,et al."An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.7(2016):1220-1224.