Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction by UHPLC-MS/MS

doi:10.1039/c6ra23808d

NWIPB OpenIR

	Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction by UHPLC-MS/MS
	Zhao, Xian-En 1,2; He, Yongrui 1,2; Yan, Ping 1,2; Wei, Na 1,2; Wang, Renjun 1,2; Sun, Jing 3,4; Zheng, Longfang 1,2; Zhu, Shuyun 1,2; You, Jinmao 1,2
	2016
发表期刊	RSC ADVANCES
卷号	6 期号:110 页码:108635-108644
文章类型	Article
摘要	A simple, rapid, sensitive and environmentally friendly ultrasound-assisted in situ derivatization dispersive liquid-liquid microextraction (in situ UA-DDLLME) method followed by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has been developed for the simultaneous determination of amino acid neurotransmitters (AANTs) and monoamine neurotransmitters (MANTs) in rat brain microdialysates and baicalein in blood microdialysates of Parkinson's disease (PD) rats with in vivo multiple sites microdialysis in this work. A novel MS sensitive derivatization reagent 4'-carbonyl chloride rosamine (CCR) with a permanent positive charged moiety, has been designed, synthesized and reported for simultaneous labelling of amino and hydroxyl groups in neurotransmitters. Under the selected conditions, the derivatization and microextraction of analytes were simultaneously completed within 1.0 min. Good linearity for each analyte (R > 0.992) was observed with a low limit of detection (LOD, S/N > 3). Taken together, in situ UA-DDLLME coupled with UHPLC-MS/MS analysis was demonstrated to be a promising method for sensitive, accurate and simultaneous monitoring of AANTs and MANTs in rat brain and baicalein in rat blood. This method would be expected to be highly useful in PD clinical diagnostics and monitoring the efficacy of treatments.
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1039/c6ra23808d
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; LC-MS/MS ; MONOAMINE NEUROTRANSMITTERS ; ELECTROCHEMICAL DETECTION ; BIOLOGICAL SAMPLES ; IONIC LIQUID ; AMINO-ACID ; CHROMATOGRAPHY ; METABOLITES ; SEROTONIN
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81303179 ; Experimental Research Project of Qufu Normal University(sj201402) ; Development Project of Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources(2014-Z-Y3) ; Open Projects Program of the Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences ; Foundation of Qufu Normal University(BSQD2012019 ; 21405094 ; 2012023) ; 31200400 ; 81403051 ; 21475075)
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000389342800059
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6657
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 4.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xian-En,He, Yongrui,Yan, Ping,et al. Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction by UHPLC-MS/MS[J]. RSC ADVANCES,2016,6(110):108635-108644.
APA	Zhao, Xian-En.,He, Yongrui.,Yan, Ping.,Wei, Na.,Wang, Renjun.,...&You, Jinmao.(2016).Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction by UHPLC-MS/MS.RSC ADVANCES,6(110),108635-108644.
MLA	Zhao, Xian-En,et al."Sensitive and accurate determination of neurotransmitters from in vivo rat brain microdialysate of Parkinson's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction by UHPLC-MS/MS".RSC ADVANCES 6.110(2016):108635-108644.