Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance

doi:10.3389/fpls.2016.02043

NWIPB OpenIR

	Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance
	Zhou, Dangwei 1,2; Yang, Yong 1; Zhang, Jinbiao 1,3; Jiang, Fei 1,4; Craft, Eric 1; Thannhauser, Theodore W.1; Kochian, Leon V.1; Liu, Jiping 1
	2017-01-09
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	7
文章类型	Article
摘要	Aluminum (Al) toxicity inhibits root growth and limits crop yields on acid soils worldwide. However, quantitative information is scarce on protein expression profiles under Al stress in crops. In this study, we report on the identification of potential Al responsive proteins from root tips of Al sensitive BR007 and Al tolerant SC566 sorghum lines using a strategy employing iTRAQ and 2D-liquid chromatography (LC) coupled to MS/MS (2D-LC-MS/MS). A total of 771 and 329 unique proteins with abundance changes of >1.5 or <0.67-told were identified in BR007 and SC566, respectively. Protein interaction and pathway analyses indicated that proteins involved in the antioxidant system were more abundant in the tolerant line than in the sensitive one after Al treatment, while opposite trends were observed for proteins involved in lignin biosynthesis. Higher levels of ROS accumulation in root tips of the sensitive line due to decreased activity of antioxidant enzymes could lead to higher lignin production and hyper-accumulation of toxic Al in cell walls. These results indicated that activities of peroxidases and the balance between production and consumption of ROS could be important for Al tolerance and lignin biosynthesis in sorghum.
关键词	Aluminum Tolerance Antioxidant System Lignin Biosynthesis Proteomics Sorghum
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fpls.2016.02043
关键词[WOS]	ACID SOILS ; MALATE TRANSPORTER ; LIGNIN DEPOSITION ; ACTIVATED CITRATE ; OXIDATIVE STRESS ; ISOBARIC TAGS ; ARABIDOPSIS ; ROOTS ; PLANTS ; RESISTANCE
收录类别	SCI
语种	英语
项目资助者	US Department of Agriculture's Agricultural Food Research Institute Grant
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000391336500002
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6664
专题	中国科学院西北高原生物研究所
作者单位	1.Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Ctr Plateau Ecol, Xining, Peoples R China 3.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Peoples R China 4.Chinese Acad Sci, Chengdu Inst Biol, Agr Biotechnol Ctr, Chengdu, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Dangwei,Yang, Yong,Zhang, Jinbiao,et al. Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance[J]. FRONTIERS IN PLANT SCIENCE,2017,7.
APA	Zhou, Dangwei.,Yang, Yong.,Zhang, Jinbiao.,Jiang, Fei.,Craft, Eric.,...&Liu, Jiping.(2017).Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance.FRONTIERS IN PLANT SCIENCE,7.
MLA	Zhou, Dangwei,et al."Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance".FRONTIERS IN PLANT SCIENCE 7(2017).