Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots | |
Zhu, Shuyun1; He, Lei1; Zhang, Fang1; Li, Mengyuan1; Jiao, Shulin1; Li, Yan1; Chen, Mengyuan1; Zhao, Xian-En1,2; Wang, Hua1 | |
2016-12-01 | |
发表期刊 | TALANTA |
卷号 | 161页码:769-774 |
文章类型 | Article |
摘要 | A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction of oxidized glutathione (GSSG) into reduced glutathione (GSH), so that the fluorescence recovery of CQDs would take place resulting from the strong GSH-Hg(II) interaction; (3) GR can lose its catalytic reduction of GSSG in the presence of its inhibitors, which will inhibit the recovery of the quenched fluorescence of CQDs. The developed CQDs-based fluorimetric method can facilitate the sensitive evaluation of GR activity in the range of 0.10-2.0 mU mL(-1) with a detection limit of 0.050 mU mL(-1). In addition, other kinds of enzymes like myoglobin, thrombin, alcohol dehyrogenase, amylase, pepsin, and trypsin could show no significant effects on the evaluation of GR activity. This work may expand the biological applications of CQDs as the fluorescent probes with low cost, easy preparation, and high photostability. |
关键词 | Carbon Quantum Dots Fluorimetric Assay Glutathione Reductase Catalysis Activity Inhibitor Screening |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1016/j.talanta.2016.09.048 |
关键词[WOS] | SELECTIVE DETECTION ; RECOVERED FLUORESCENCE ; ALKALINE-PHOSPHATASE ; DOTS-HG(II) SYSTEM ; ASSAY ; BIOTHIOLS ; NANODOTS ; GREEN ; ACID ; NANOCLUSTERS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2013BQ018) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Student Research Training Program of Qufu Normal University(2015A071) ; Scientific Research Fundation of Qufu Normal University(bsqd2012023) ; Taishan Scholar Foundation of Shandong Province, China ; 81303179 ; 21375075) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000386989500101 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6666 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Shuyun,He, Lei,Zhang, Fang,et al. Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots[J]. TALANTA,2016,161:769-774. |
APA | Zhu, Shuyun.,He, Lei.,Zhang, Fang.,Li, Mengyuan.,Jiao, Shulin.,...&Wang, Hua.(2016).Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots.TALANTA,161,769-774. |
MLA | Zhu, Shuyun,et al."Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots".TALANTA 161(2016):769-774. |
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