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Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots
Zhu, Shuyun1; He, Lei1; Zhang, Fang1; Li, Mengyuan1; Jiao, Shulin1; Li, Yan1; Chen, Mengyuan1; Zhao, Xian-En1,2; Wang, Hua1
2016-12-01
发表期刊TALANTA
卷号161页码:769-774
文章类型Article
摘要A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction of oxidized glutathione (GSSG) into reduced glutathione (GSH), so that the fluorescence recovery of CQDs would take place resulting from the strong GSH-Hg(II) interaction; (3) GR can lose its catalytic reduction of GSSG in the presence of its inhibitors, which will inhibit the recovery of the quenched fluorescence of CQDs. The developed CQDs-based fluorimetric method can facilitate the sensitive evaluation of GR activity in the range of 0.10-2.0 mU mL(-1) with a detection limit of 0.050 mU mL(-1). In addition, other kinds of enzymes like myoglobin, thrombin, alcohol dehyrogenase, amylase, pepsin, and trypsin could show no significant effects on the evaluation of GR activity. This work may expand the biological applications of CQDs as the fluorescent probes with low cost, easy preparation, and high photostability.
关键词Carbon Quantum Dots Fluorimetric Assay Glutathione Reductase Catalysis Activity Inhibitor Screening
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.talanta.2016.09.048
关键词[WOS]SELECTIVE DETECTION ; RECOVERED FLUORESCENCE ; ALKALINE-PHOSPHATASE ; DOTS-HG(II) SYSTEM ; ASSAY ; BIOTHIOLS ; NANODOTS ; GREEN ; ACID ; NANOCLUSTERS
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2013BQ018) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Student Research Training Program of Qufu Normal University(2015A071) ; Scientific Research Fundation of Qufu Normal University(bsqd2012023) ; Taishan Scholar Foundation of Shandong Province, China ; 81303179 ; 21375075)
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000386989500101
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6666
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
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Zhu, Shuyun,He, Lei,Zhang, Fang,et al. Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots[J]. TALANTA,2016,161:769-774.
APA Zhu, Shuyun.,He, Lei.,Zhang, Fang.,Li, Mengyuan.,Jiao, Shulin.,...&Wang, Hua.(2016).Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots.TALANTA,161,769-774.
MLA Zhu, Shuyun,et al."Fluorimetric evaluation of glutathione reductase activity and its inhibitors using carbon quantum dots".TALANTA 161(2016):769-774.
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