First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography

doi:10.1002/jssc.201600805

NWIPB OpenIR

	First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography
	Gong, Chengcheng 1; Chen, Tao 2; Chen, Huifeng 1; Zhang, Shanshan 1; Wang, Xinzhu 1; Wang, Weiqing 3; Sun, Jing 2; Li, Yulin 2; Liao, Zhixin 1
	2016-12-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	39 期号:23 页码:4660-4666
文章类型	Article
摘要	In this paper, we report an efficient method by high-speed counter-current chromatography for the first separation of four aromatic acids and two analogs with similar structures and polarities from Clematis akebioides. First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high-speed counter-counter chromatography using a two-phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high-speed counter-current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from Clematis akebioides.
关键词	Aromatic Acids Clematis Akebioides High-speed Counter-counter Chromatography
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201600805
关键词[WOS]	PREPARATIVE SEPARATION ; CHEMICAL-CONSTITUENTS ; RHEUM-TANGUTICUM ; PURIFICATION ; EXTRACTION ; FLAVONOIDS ; HPLC
收录类别	SCI
语种	英语
项目资助者	priority academic program development of Jiangsu higher Education Institutions(1107047002) ; National Natural Science Foundation of China(81403051) ; Instrument Function Development Project of CAS(2017g107)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000393030100023
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6781
专题	中国科学院西北高原生物研究所
作者单位	1.Southeast Univ, Sch Chem & Chem Engn, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 3.Qinghai Prov Prod Qual Supervis & Inspect Ctr, Xining, Peoples R China
推荐引用方式 GB/T 7714	Gong, Chengcheng,Chen, Tao,Chen, Huifeng,et al. First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(23):4660-4666.
APA	Gong, Chengcheng.,Chen, Tao.,Chen, Huifeng.,Zhang, Shanshan.,Wang, Xinzhu.,...&Liao, Zhixin.(2016).First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,39(23),4660-4666.
MLA	Gong, Chengcheng,et al."First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 39.23(2016):4660-4666.