Frame-shift Mutation Causes the Function Loss of TaMYB-A1 Regulating Anthocyanin Biosynthesis in Triticum aestivum

doi:10.1556/0806.44.2016.042

NWIPB OpenIR

	Frame-shift Mutation Causes the Function Loss of TaMYB-A1 Regulating Anthocyanin Biosynthesis in Triticum aestivum
	Ye, G. J.1,2; Wei, L.1,3; Chen, W. J.1,3; Zhang, B.1,3; Liu, B. L.1,3; Zhang, H. G.1,3
	2017-03-01
发表期刊	CEREAL RESEARCH COMMUNICATIONS
卷号	45 期号:1 页码:35-46
文章类型	Article
摘要	Red coleoptile is an easily observed trait in Triticum aestivum and can provide some protection against stress. Here, TaMYB-A1 or TuMYB-A1, homologous to TaMYB-D1, which controls red coleoptile formation in the common wheat cultivar 'Gy115', was isolated from eight T. aestivum and 34 T. urartu cultivars. The genome sequence of TaMYB-A1 was 867 bp with an intron of 93 bp, which was similar to the MYBs regulating anthocyanin biosynthesis in T. aestivum but different from other MYB transcription factors regulating anthocyanin biosynthesis. TaMYB-A1 had an integrated DNA-binding domain of 102 amino acids and a transcriptional domain of 42 amino acids, which was responsible for regulating anthocyanin biosynthesis. TaMYB-A1 was assigned to the same branch as the MYBs regulating anthocyanin biosynthesis in a phylogenetic tree. A transient expression analysis showed that TaMYB-A1 induced 'Opata' coleoptile cells to synthesize anthocyanin with the help of ZmR. A non-functional allele of TaMYB-a1 existed in common wheat cultivars containing rc-a1. One single nucleotide was deleted 715 bp after the start codon in TaMYB-a1 compared with TaMYB-A1. The deletion caused a frame shift mutation, destroyed the DNA transcription activator domain, and resulted in TaMYB-a1 losing its ability to regulate anthocyanin biosynthesis in 'Opata' coleoptile cells. Those cultivars with functional TaMYB-A1 or TuMYB-A1 have red coleoptiles. The isolation of TaMYB-A1 should aid in understanding the molecular mechanisms of coleoptile traits in T. aestivum.
关键词	Triticum Aestivum Red Coleoptile Myb Transcription Factor Function Verification
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1556/0806.44.2016.042
关键词[WOS]	MOLECULAR ANALYSIS ; WHEAT COLEOPTILE ; GRAIN COLOR ; MYB DOMAIN ; RED-LIGHT ; MAIZE C1 ; GENES ; ACCUMULATION ; EXPRESSION ; RESPONSES
收录类别	SCI
语种	英语
项目资助者	Basic Research Projects of Qinghai Province(2015-ZJ-701) ; Pilot Projects of Designer Breeding by Molecular Modules, Chinese Academy of Sciences ; National Natural Science Foundation of China(31071417 ; West Light Foundation of the Chinese Academy of Sciences ; 31260322)
WOS研究方向	Agriculture
WOS类目	Agronomy
WOS记录号	WOS:000394551500003
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6791
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Ye, G. J.,Wei, L.,Chen, W. J.,et al. Frame-shift Mutation Causes the Function Loss of TaMYB-A1 Regulating Anthocyanin Biosynthesis in Triticum aestivum[J]. CEREAL RESEARCH COMMUNICATIONS,2017,45(1):35-46.
APA	Ye, G. J.,Wei, L.,Chen, W. J.,Zhang, B.,Liu, B. L.,&Zhang, H. G..(2017).Frame-shift Mutation Causes the Function Loss of TaMYB-A1 Regulating Anthocyanin Biosynthesis in Triticum aestivum.CEREAL RESEARCH COMMUNICATIONS,45(1),35-46.
MLA	Ye, G. J.,et al."Frame-shift Mutation Causes the Function Loss of TaMYB-A1 Regulating Anthocyanin Biosynthesis in Triticum aestivum".CEREAL RESEARCH COMMUNICATIONS 45.1(2017):35-46.