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Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction
Sun, Jing1,2; Zhao, Xian-En1,2,3,4; Dang, Jun1,2; Sun, Xiaoyan3,4; Zheng, Longfang3,4; You, Jinmao3,4; Wang, Xiao5
2017-02-01
发表期刊JOURNAL OF SEPARATION SCIENCE
卷号40期号:3页码:725-732
文章类型Article
摘要In this work, a hyphenated technique of dual ultrasound-assisted dispersive liquid-liquid microextraction combined with microwave-assisted derivatization followed by ultra high performance liquid chromatography tandem mass spectrometry has been developed for the determination of phytosterols in functional foods and medicinal herbs. Multiple reaction monitoring mode was used for the tandem mass spectrometry detection. A mass spectrometry sensitive reagent, 4'-carboxy-substituted rosamine, has been used as the derivatization reagent for five phytosterols, and internal standard diosgenin was used for the first time. Parameters for the dual microextraction, microwave-assisted derivatization, and ultra high performance liquid chromatography tandem mass spectrometry were all optimized in detail. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect, extremely low limits of detection (0.005-0.015 ng/mL) and limits of quantification (0.030-0.10 ng/mL) were achieved. The proposed methodwas compared with previously reported methods. It showed better sensitivity, selectivity, and accuracy. The matrix effect was also significantly reduced. The proposed method was successfully applied to the determination of five phytosterols in vegetable oil (sunflower oil, olive oil, corn oil, peanut oil), milk and orange juice (soymilk, peanut milk, orange juice), and medicinal herbs (Ginseng, Ganoderma lucidum, Cordyceps, Polygonum multiflorum) for the quality control of functional foods and medicinal herbs.
关键词Derivatization Dispersive Liquid-liquid Microextraction Phytosterol Sensitivity Enhancement Triple Quadrupole Mass Spectrometry
WOS标题词Science & Technology ; Physical Sciences
DOI10.1002/jssc.201600711
关键词[WOS]TANDEM MASS-SPECTROMETRY ; SUPERCRITICAL CARBON-DIOXIDE ; FLUORESCENCE DETECTION ; CHROMATOGRAPHIC METHOD ; PHASE MICROEXTRACTION ; GAS-CHROMATOGRAPHY ; DAIRY-PRODUCTS ; WATER SAMPLES ; RHODAMINE-B ; EXTRACTION
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(81403051 ; National Training Program of Innovation and Entrepreneurship for Undergraduates(201610446031) ; Development Project of Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources(2014-Z-Y3) ; Open Funds of Key Laboratory of TCM Quality Control Technology(2016003) ; 81303179 ; 21405094)
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000397029500013
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6800
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Qinghai, Peoples R China
3.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China
4.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharm Intermediates & Anal Nat Med, Qufu, Shandong, Peoples R China
5.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, Jinan, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Sun, Jing,Zhao, Xian-En,Dang, Jun,et al. Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(3):725-732.
APA Sun, Jing.,Zhao, Xian-En.,Dang, Jun.,Sun, Xiaoyan.,Zheng, Longfang.,...&Wang, Xiao.(2017).Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction.JOURNAL OF SEPARATION SCIENCE,40(3),725-732.
MLA Sun, Jing,et al."Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction".JOURNAL OF SEPARATION SCIENCE 40.3(2017):725-732.
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