Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction | |
Sun, Jing1,2; Zhao, Xian-En1,2,3,4; Dang, Jun1,2; Sun, Xiaoyan3,4; Zheng, Longfang3,4; You, Jinmao3,4; Wang, Xiao5 | |
2017-02-01 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE |
卷号 | 40期号:3页码:725-732 |
文章类型 | Article |
摘要 | In this work, a hyphenated technique of dual ultrasound-assisted dispersive liquid-liquid microextraction combined with microwave-assisted derivatization followed by ultra high performance liquid chromatography tandem mass spectrometry has been developed for the determination of phytosterols in functional foods and medicinal herbs. Multiple reaction monitoring mode was used for the tandem mass spectrometry detection. A mass spectrometry sensitive reagent, 4'-carboxy-substituted rosamine, has been used as the derivatization reagent for five phytosterols, and internal standard diosgenin was used for the first time. Parameters for the dual microextraction, microwave-assisted derivatization, and ultra high performance liquid chromatography tandem mass spectrometry were all optimized in detail. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect, extremely low limits of detection (0.005-0.015 ng/mL) and limits of quantification (0.030-0.10 ng/mL) were achieved. The proposed methodwas compared with previously reported methods. It showed better sensitivity, selectivity, and accuracy. The matrix effect was also significantly reduced. The proposed method was successfully applied to the determination of five phytosterols in vegetable oil (sunflower oil, olive oil, corn oil, peanut oil), milk and orange juice (soymilk, peanut milk, orange juice), and medicinal herbs (Ginseng, Ganoderma lucidum, Cordyceps, Polygonum multiflorum) for the quality control of functional foods and medicinal herbs. |
关键词 | Derivatization Dispersive Liquid-liquid Microextraction Phytosterol Sensitivity Enhancement Triple Quadrupole Mass Spectrometry |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1002/jssc.201600711 |
关键词[WOS] | TANDEM MASS-SPECTROMETRY ; SUPERCRITICAL CARBON-DIOXIDE ; FLUORESCENCE DETECTION ; CHROMATOGRAPHIC METHOD ; PHASE MICROEXTRACTION ; GAS-CHROMATOGRAPHY ; DAIRY-PRODUCTS ; WATER SAMPLES ; RHODAMINE-B ; EXTRACTION |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(81403051 ; National Training Program of Innovation and Entrepreneurship for Undergraduates(201610446031) ; Development Project of Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources(2014-Z-Y3) ; Open Funds of Key Laboratory of TCM Quality Control Technology(2016003) ; 81303179 ; 21405094) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000397029500013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6800 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Qinghai, Peoples R China 3.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharm Intermediates & Anal Nat Med, Qufu, Shandong, Peoples R China 5.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, Jinan, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Jing,Zhao, Xian-En,Dang, Jun,et al. Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(3):725-732. |
APA | Sun, Jing.,Zhao, Xian-En.,Dang, Jun.,Sun, Xiaoyan.,Zheng, Longfang.,...&Wang, Xiao.(2017).Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction.JOURNAL OF SEPARATION SCIENCE,40(3),725-732. |
MLA | Sun, Jing,et al."Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction".JOURNAL OF SEPARATION SCIENCE 40.3(2017):725-732. |
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