ID4 levels dictate the stem cell state in mouse spermatogonia

doi:10.1242/dev.146928

NWIPB OpenIR

	ID4 levels dictate the stem cell state in mouse spermatogonia
	Helsel, Aileen R.1; Yang, Qi-En 1,2; Oatley, Melissa J.1; Lord, Tessa 1; Sablitzky, Fred 3; Oatley, Jon M.1
	2017-02-15
发表期刊	DEVELOPMENT
卷号	144 期号:4 页码:624-634
文章类型	Article
摘要	Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and the formation of progenitors as the initial step in the production of differentiated cells. The mechanisms that guide the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets, with the ID4-EGFPBright population being mostly, if not purely, SSCs, whereas the ID4EGFPDim population is in transition to the progenitor state. These subsets are also distinguishable by transcriptome signatures. Moreover, using a conditional overexpression mouse model, we found that transition from the stem cell to the immediate progenitor state requires downregulation of Id4 coincident with a major change in the transcriptome. Collectively, our results demonstrate that the level of ID4 is predictive of stem cell or progenitor capacity in spermatogonia and dictates the interface of transition between the different functional states.
关键词	Id4 Progenitor Spermatogonia Stem Cell
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1242/dev.146928
关键词[WOS]	SELF-RENEWAL ; MALE GERMLINE ; EXPRESSION ; TESTES ; PROTEIN ; LINE ; SPERMATOGENESIS ; TRANSPLANTATION ; TRANSCRIPTION ; MICE
收录类别	SCI
语种	英语
项目资助者	Eunice Kennedy Shriver National Institute of Child Health and Human Development(HD061665)
WOS研究方向	Developmental Biology
WOS类目	Developmental Biology
WOS记录号	WOS:000394381100009
引用统计	被引频次：130[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6804
专题	中国科学院西北高原生物研究所
作者单位	1.Washington State Univ, Coll Vet Med, Ctr Reprod Biol, Sch Mol Biosci, Pullman, WA 99164 USA 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, QH, Peoples R China 3.Univ Nottingham, Sch Life Sci, Nottingham NG7 2UH, England
推荐引用方式 GB/T 7714	Helsel, Aileen R.,Yang, Qi-En,Oatley, Melissa J.,et al. ID4 levels dictate the stem cell state in mouse spermatogonia[J]. DEVELOPMENT,2017,144(4):624-634.
APA	Helsel, Aileen R.,Yang, Qi-En,Oatley, Melissa J.,Lord, Tessa,Sablitzky, Fred,&Oatley, Jon M..(2017).ID4 levels dictate the stem cell state in mouse spermatogonia.DEVELOPMENT,144(4),624-634.
MLA	Helsel, Aileen R.,et al."ID4 levels dictate the stem cell state in mouse spermatogonia".DEVELOPMENT 144.4(2017):624-634.