Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS

doi:10.1080/01496395.2017.1287738

NWIPB OpenIR

	Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS
	Chen, Tao 1,2; Li, Hongmei 1,2,3; Tan, Liang 1,2; Li, Yulin 1,2
	2017
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
卷号	52 期号:8 页码:1423-1429
文章类型	Article
摘要	A minor lignan, 4'-demethyldeoxypodophyllotoxin, was isolated from Sinopodophyllum emodi. First, a crude extract of S. emodi was analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS) to confirm the presence of the target compound. Then, the target lignan was successfully enriched in the crude extract precipitate by medium-pressure LC (MPLC) with high recovery (85%). Finally, an efficient separation was achieved by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v/v/v). These results clearly demonstrate that the combination of HPLC-MS, MPLC, and HSCCC could be a powerful technique for the rapid screening, enrichment, and separation of minor compounds from complex natural products.
关键词	4 '-demethyldeoxypodophyllotoxin High-speed Counter-current Chromatography Hplc-ms Minor Lignan Sinopodophyllum Emodi
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1080/01496395.2017.1287738
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MACROPOROUS RESIN ; MASS-SPECTROMETRY ; RHEUM-TANGUTICUM ; RAPID ENRICHMENT ; NATURAL-PRODUCTS ; PURIFICATION ; EXTRACTION ; GLYCOSIDES ; LIGNANS
收录类别	SCI
语种	英语
项目资助者	Personalized Medicines-Molecular Signature-based Drug Discovery and Development, Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12050304) ; General Program of Natural Science Foundation of Qinghai Province(2015-ZJ-908) ; Instrument Function Development Project of CAS(2017g107)
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000399716200011
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6815
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 3.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, P. R. China
推荐引用方式 GB/T 7714	Chen, Tao,Li, Hongmei,Tan, Liang,et al. Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS[J]. SEPARATION SCIENCE AND TECHNOLOGY,2017,52(8):1423-1429.
APA	Chen, Tao,Li, Hongmei,Tan, Liang,&Li, Yulin.(2017).Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS.SEPARATION SCIENCE AND TECHNOLOGY,52(8),1423-1429.
MLA	Chen, Tao,et al."Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS".SEPARATION SCIENCE AND TECHNOLOGY 52.8(2017):1423-1429.