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Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis
Lv, Zhengxian1,2; You, Jinmao1,2,3; Lu, Shuaimin1,2; Sun, Weidi1; Ji, Zhongyin2; Sun, Zhiwei1,2; Song, Cuihua1,2; Chen, Guang1,2; Li, Guoliang1; Hu, Na3; Zhou, Wu4; Suo, Yourui3
2017-03-31
发表期刊JOURNAL OF CHROMATOGRAPHY A
卷号1491页码:98-107
文章类型Article
摘要As the key aroma compounds, varietal thiols are the crucial odorants responsible for the flavor of wines. Quantitative analysis of thiols can provide crucial information for the aroma profiles of different wine styles. In this study, a rapid and sensitive method for the simultaneous determination of six thiols in wine using d(0)/d(4)-acridone-10-ethyl-N-maleimide (d(0)/d(4)-AENM) as stable isotope-coded derivatization reagent (SICD) by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) has been developed. Quantification of thiols was performed by using d(4)-AENM labeled thiols as the internal standards (IS), followed by stable isotope dilution HPLC-ESIMS/MS analysis. The AENM derivatization combined with multiple reactions monitoring (MRM) not only allowed trace analysis of thiols due to the extremely high sensitivity, but also efficiently corrected the matrix effects during HPLC-MS/MS and the fluctuation in MS/MS signal intensity due to instrument. The obtained internal standard calibration curves for six thiols were linear over the range of 25-10,000 pmol/L (R-2 >= 0.9961). Detection limits (LODs) for most of analytes were below 63 pmol/L. The proposed method was successfully applied for the simultaneous determination of six kinds of thiols in wine samples with precisions <3.5% and recoveries >78.1%. In conclusion, the developed method is expected to be a promising tool for detection of trace thiols in wine and also in other complex matrix. (C) 2017 Elsevier B.V. All rights reserved.
关键词Stable Isotope-coded Derivatization (Sicd) Hplc-esi-ms/ms Thiols Wine
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.02.042
关键词[WOS]SOLID-PHASE MICROEXTRACTION ; VINIFERA GRAPE VARIETIES ; QUANTITATIVE-DETERMINATION ; 3-MERCAPTOHEXYL ACETATE ; VOLATILE THIOLS ; CHROMATOGRAPHY/MASS SPECTROMETRY ; POLYFUNCTIONAL MERCAPTANS ; FLUORESCENCE DETECTION ; INTERNAL STANDARDS ; SAUVIGNON BLANC
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21475075 ; National Natural Science Foundation of Shandong Province(ZR2015CM040) ; 21475074 ; 21305076 ; 21275089)
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000397375700011
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6827
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med S, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
4.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China
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GB/T 7714
Lv, Zhengxian,You, Jinmao,Lu, Shuaimin,et al. Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1491:98-107.
APA Lv, Zhengxian.,You, Jinmao.,Lu, Shuaimin.,Sun, Weidi.,Ji, Zhongyin.,...&Suo, Yourui.(2017).Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis.JOURNAL OF CHROMATOGRAPHY A,1491,98-107.
MLA Lv, Zhengxian,et al."Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis".JOURNAL OF CHROMATOGRAPHY A 1491(2017):98-107.
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