NWIPB OpenIR
Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis
Lv, Zhengxian1,2; You, Jinmao1,2,3; Lu, Shuaimin1,2; Sun, Weidi1; Ji, Zhongyin2; Sun, Zhiwei1,2; Song, Cuihua1,2; Chen, Guang1,2; Li, Guoliang1; Hu, Na3; Zhou, Wu4; Suo, Yourui3
2017-03-31
Source PublicationJOURNAL OF CHROMATOGRAPHY A
Volume1491Pages:98-107
SubtypeArticle
AbstractAs the key aroma compounds, varietal thiols are the crucial odorants responsible for the flavor of wines. Quantitative analysis of thiols can provide crucial information for the aroma profiles of different wine styles. In this study, a rapid and sensitive method for the simultaneous determination of six thiols in wine using d(0)/d(4)-acridone-10-ethyl-N-maleimide (d(0)/d(4)-AENM) as stable isotope-coded derivatization reagent (SICD) by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) has been developed. Quantification of thiols was performed by using d(4)-AENM labeled thiols as the internal standards (IS), followed by stable isotope dilution HPLC-ESIMS/MS analysis. The AENM derivatization combined with multiple reactions monitoring (MRM) not only allowed trace analysis of thiols due to the extremely high sensitivity, but also efficiently corrected the matrix effects during HPLC-MS/MS and the fluctuation in MS/MS signal intensity due to instrument. The obtained internal standard calibration curves for six thiols were linear over the range of 25-10,000 pmol/L (R-2 >= 0.9961). Detection limits (LODs) for most of analytes were below 63 pmol/L. The proposed method was successfully applied for the simultaneous determination of six kinds of thiols in wine samples with precisions <3.5% and recoveries >78.1%. In conclusion, the developed method is expected to be a promising tool for detection of trace thiols in wine and also in other complex matrix. (C) 2017 Elsevier B.V. All rights reserved.
KeywordStable Isotope-coded Derivatization (Sicd) Hplc-esi-ms/ms Thiols Wine
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.02.042
WOS KeywordSOLID-PHASE MICROEXTRACTION ; VINIFERA GRAPE VARIETIES ; QUANTITATIVE-DETERMINATION ; 3-MERCAPTOHEXYL ACETATE ; VOLATILE THIOLS ; CHROMATOGRAPHY/MASS SPECTROMETRY ; POLYFUNCTIONAL MERCAPTANS ; FLUORESCENCE DETECTION ; INTERNAL STANDARDS ; SAUVIGNON BLANC
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21475075 ; National Natural Science Foundation of Shandong Province(ZR2015CM040) ; 21475074 ; 21305076 ; 21275089)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000397375700011
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/6827
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med S, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
4.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China
Recommended Citation
GB/T 7714
Lv, Zhengxian,You, Jinmao,Lu, Shuaimin,et al. Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1491:98-107.
APA Lv, Zhengxian.,You, Jinmao.,Lu, Shuaimin.,Sun, Weidi.,Ji, Zhongyin.,...&Suo, Yourui.(2017).Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis.JOURNAL OF CHROMATOGRAPHY A,1491,98-107.
MLA Lv, Zhengxian,et al."Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis".JOURNAL OF CHROMATOGRAPHY A 1491(2017):98-107.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lv, Zhengxian]'s Articles
[You, Jinmao]'s Articles
[Lu, Shuaimin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lv, Zhengxian]'s Articles
[You, Jinmao]'s Articles
[Lu, Shuaimin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lv, Zhengxian]'s Articles
[You, Jinmao]'s Articles
[Lu, Shuaimin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.