Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS

doi:10.1016/j.jpba.2016.11.056

NWIPB OpenIR

	Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS
	Zhao, Xian-En 1,2; He, Yongrui 1,2; Li, Meng 1,2; Chen, Guang 1,2; Wei, Na 1,2; Wang, Xiao 5; Sun, Jing 3,4; Zhu, Shuyun 1,2; You, Jinmao 1,2
	2017-02-20
发表期刊	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷号	135 页码:186-198
文章类型	Article
摘要	Neurotransmitters (NTs) may play an important role in neuro degenerative disorders such as Alzheimer's disease (AD). In order to investigate the potential links, a new simple, fast, accurate and sensitive analytical method, based on in situ ultrasound-assisted derivatization dispersive liquid-liquid microex-traction (in situ UA-DDLLME) coupled with ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), has been developed and validated. The quantitation of amino acid neu-rotransmitters (AANTs) and monoamine neurotransmitters (MANTs) in urine of AD rats were performed in this work. The in situ UA-DDLLME procedure involved the rapid injection of the mixture of low toxic 4-bromoanisole (extractant) and acetonitrile (dispersant), which containing the new designed and synthesized 4'-carbonyl chloride rosamine (CCR) as derivatization reagent, into the aqueous phase of real sample and buffer. Under the selected conditions, the derivatization and microextraction of analytes were simultaneously completed within 1 min. Good linearity for each analyte (R > 0.992) was observed with low limit of detections (LODs, S/N > 3). Moreover, the proposed method was compared with direct detection or other reported methods, and the results showed that low matrix effects and good recoveries results were obtained in this work. Taken together, in situ UA-DDLLME coupled with UHPLC-MS/MS analysis was demonstrated to be a good method for sensitive, accurate and simultaneous monitoring of AANTs and MANTs. This method would be expected to be highly useful in AD diseases' clinical diagnostics and may have potential value in monitoring the efficacy of treatment. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Neurotransmitter In Situ Derivatization Ultrasound-assisted Dispersive Liquid-liquid Microextraction Uhplc-ms/ms Alzheimer's Diseases Urine
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI	10.1016/j.jpba.2016.11.056
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; LC-MS/MS ; SIMULTANEOUS QUANTIFICATION ; MULTIPLE NEUROTRANSMITTERS ; CHROMATOGRAPHY ; BRAIN ; DOPAMINE ; MICRODIALYSIS ; EXTRACTION ; SAMPLES
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81303179 ; Experimental Research Project of Qufu Normal University(sj201402) ; Open Funds of Key Laboratory of TCM Quality Control Technology(2016003) ; Open Projects Program of the Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences ; Foundation of Qufu Normal University(BSQD2012019 ; 81403051 ; BSQD2012023) ; 21475074 ; 21405094 ; 21475075)
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
WOS记录号	WOS:000392905200021
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6832
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 5.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xian-En,He, Yongrui,Li, Meng,et al. Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,135:186-198.
APA	Zhao, Xian-En.,He, Yongrui.,Li, Meng.,Chen, Guang.,Wei, Na.,...&You, Jinmao.(2017).Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,135,186-198.
MLA	Zhao, Xian-En,et al."Analysis of amino acid and monoamine neurotransmitters and their metabolites in rat urine of Alzheimer's disease using in situ ultrasound-assisted derivatization dispersive liquid-liquid microextraction with UHPLC-MS/MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 135(2017):186-198.