Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

doi:10.1128/AEM.02319-16

NWIPB OpenIR

	Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae
	Fang, Zhijia 1; Chen, Zhongxiang 1; Wang, Song 2; Shi, Ping 2; Shen, Yuhu 3; Zhang, Youshang 1; Xiao, Junhua 1; Huang, Zhiwei 1
	2017
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号	83 期号:1
文章类型	Article
摘要	The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. Saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. We investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmium-stress activity. The results demonstrated that the expression of OLE1 was positively correlated with cadmium stress tolerance and induction was independent of Mga2p and Spt23p (important regulatory factors for OLE1). Moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. The addition of exogenous unsaturated fatty acids simulated overexpression of OLE1, leading to cadmium resistance. Such regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium.
关键词	Cadmium Ole1 Delta-9 Desaturase Unsaturated Fatty Acids Cytoplasmic Membrane
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1128/AEM.02319-16
关键词[WOS]	STEAROYL-COA DESATURASE ; UNSATURATED FATTY-ACID ; LIPID-COMPOSITION ; PLASMA-MEMBRANE ; GENE-EXPRESSION ; CELL-DEATH ; YEAST ; TOLERANCE ; FLUIDITY ; STRESS
收录类别	SCI
语种	英语
项目资助者	Natural Science Foundation of Shanghai(15ZR1400200) ; Shanghai Scientific and Technological Innovation Project(14520720700) ; State Education Ministry ; Fundamental Research Funds for the Central Universities(2232014A3-03 ; Applied Basic Research Project of Promotion Plan for Scientific and Technological Innovation in Qinghai Province(2015-ZJ-703) ; 222201313010)
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000393205400006
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6833
专题	中国科学院西北高原生物研究所
作者单位	1.Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai, Peoples R China 2.East China Univ Sci & Technol, Key Lab Bioreactor Engn, Shanghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai Provinc, Peoples R China
推荐引用方式 GB/T 7714	Fang, Zhijia,Chen, Zhongxiang,Wang, Song,et al. Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(1).
APA	Fang, Zhijia.,Chen, Zhongxiang.,Wang, Song.,Shi, Ping.,Shen, Yuhu.,...&Huang, Zhiwei.(2017).Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(1).
MLA	Fang, Zhijia,et al."Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.1(2017).