NWIPB OpenIR
Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification
Zhao, X; Li, YL; Suo, YR; Shi, YW; Chen, XM; Zhang, HF; Sun, XJ; You, JM
2006-02-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号34期号:2页码:150-154
文章类型Article
摘要A simple and sensitive method for the determination of free fatty acids using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescence derivatization reagent by reversed-phase high-performance liquid chromatography has been developed. Studies on derivatization conditions indicate that free fatty acids react rapidly and smoothly with AETS in the presence of K2CO3 catalyst at 85 degrees C in N,N-dimethylformamide solvent to give the corresponding sensitively fluorescent derivatives with maximal yields close to 100% with a 6-fold molar reagent excess. Free fatty acid derivatives were separated on Eclipse XDB-C-8 column with a good baseline resolution. The identification of 19 fatty acid derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source under positive-ion mode detection. The contents of 19 free fatty acids in soil and three kinds of bryophytes (tree moss, entodon macropodus, dicranum caesium) were determined. The results indicate that the bryophyte plants enrich an abundance of free fatty acids from soil. The fluorescence excitation and emission wavelengths of derivatives were set at lambda(ex)=404 and lambda(em)=440 nm, respectively. Most linear correlation coefficients for fatty acid derivatives are over 0.9996, and detection limits (at signal-to-noise of 3:1) are 12.28 similar to 43.69 fmol.
关键词High Performance Liquid Chromatography/mass Spectrometry Fluorescence Detection Derivatization Fatty Acids
WOS标题词Science & Technology ; Physical Sciences
关键词[WOS]LABELING REAGENT ; DERIVATIVES
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000235798500002
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6858
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714
Zhao, X,Li, YL,Suo, YR,et al. Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(2):150-154.
APA Zhao, X.,Li, YL.,Suo, YR.,Shi, YW.,Chen, XM.,...&You, JM.(2006).Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(2),150-154.
MLA Zhao, X,et al."Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.2(2006):150-154.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, X]的文章
[Li, YL]的文章
[Suo, YR]的文章
百度学术
百度学术中相似的文章
[Zhao, X]的文章
[Li, YL]的文章
[Suo, YR]的文章
必应学术
必应学术中相似的文章
[Zhao, X]的文章
[Li, YL]的文章
[Suo, YR]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。