Determination of steroids from rat feces by high performance liquid chromatography with fluorescence derivatization and identification by on-line mass spectrometry

NWIPB OpenIR

	Determination of steroids from rat feces by high performance liquid chromatography with fluorescence derivatization and identification by on-line mass spectrometry
	Chen Wandong ; Zhang Haifeng ; Guo Qingjun ; Wang Xianfeng ; Suo Yourui ; Chen Guichen ; Zhang Honghai ; You Jinmao
	2006-10-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	34 期号:10 页码:1406-1410
文章类型	Article
摘要	A simple and sensitive method for the determination of steroid compounds extracted from rat feces using 1 2-benzo-3 4-dihydrocarbazole-9-ethyl-carbonylhydazine (BCEC) as pre-column derivatization reagent by high performance liquid chromatography in combination with a gradient elution with fluorescence detection and mass spectrometric identification has been developed. Studies. on derivatization conditions indicate that steroid compounds react with BCEC at 65 degrees C within a two-hour period in the presence of trichloroacetic acid (TCA) catalyst with acetonitrile as reaction co-solvent to give the corresponding sensitive fluorescence derivatives. The fluorescence excitation and emission wavelengths are 333 nm and 390 nm, respectively. The identification of steroid derivatives is performed under atmospheric-pressure chemical ionization (APCI) source in positive-ion mode.. Correlation coefficients for steroid derivatives are > 0.9999, and detection limits (at signal-to-noise ratio of 3 : 1) are between 47.3 to 71.2 fmol. The established method is sensitive and reproducible for the determination of steroid compounds from real samples with satisfactory results.
关键词	High Performance Liquid Chromatography/mass Spectrometry Fluorescence Derivatization Pre-column Derivatization Steroids
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	GLUCOCORTICOIDS ; VALIDATION ; STRESS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000243502600010
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6859
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Qufu Normal Univ, Coll Life Sci, Qufu 273165, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 4.Jining Teachers Coll, Jining 272025, Peoples R China
推荐引用方式 GB/T 7714	Chen Wandong,Zhang Haifeng,Guo Qingjun,et al. Determination of steroids from rat feces by high performance liquid chromatography with fluorescence derivatization and identification by on-line mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(10):1406-1410.
APA	Chen Wandong.,Zhang Haifeng.,Guo Qingjun.,Wang Xianfeng.,Suo Yourui.,...&You Jinmao.(2006).Determination of steroids from rat feces by high performance liquid chromatography with fluorescence derivatization and identification by on-line mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(10),1406-1410.
MLA	Chen Wandong,et al."Determination of steroids from rat feces by high performance liquid chromatography with fluorescence derivatization and identification by on-line mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.10(2006):1406-1410.