Pre-column derivatization-high performance liquid chromatography for the determination of aliphatic amines with fluorescence detection and mass spectrometric identification

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	Pre-column derivatization-high performance liquid chromatography for the determination of aliphatic amines with fluorescence detection and mass spectrometric identification
	Zhao Xian-En ; Li Yu-Lin ; You Jin-Mao ; Liu Yong-Jun ; Suo You-Rui
	2007-06-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	35 期号:6 页码:779-785
文章类型	Article
摘要	A simple and highly sensitive method based on the derivatization of aliphatic amines with 2-(2-phenyl-1 H-phenanthro-[9, 10-d] imidazole-1-yl)-acetic acid (PPIA) followed by high performance liquid chromatography with fluorescence detection and on-line post-column mass spectrometric identification has been developed. Optimum derivatization, giving the corresponding stable fluorescent derivatives, was obtained by reacting of aliphatic amines with PPIA at 60 degrees C for 15 min in the presence of N-ethyl-N'-(3-dimethylamino propyl) carbodiimide hydrochloride (EDC) catalyst in acetonitrile solvent. The fluorescence detection Wavelength of PPIA derivatives was 380 nm (excitation wavelength 260 nm). Separation of the derivatized amines with a good baseline resolution in conjunction with a gradient elution was carried out on a reversed phase Eclipse XDB-C-8 column (4.6 min x 150 min, 5 mu m), in this way, the content of free fatty amines in waste water of paper mill, telencephalon tissue of rat and acidophilus milk was determined. The identification of aliphatic amine derivatives was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization source under positive-ion mode detection. The established method exhibits excellent reproducibility and recovery. Excellent linear responses are observed with regression coefficients > 0.9996, detection limits ( at signal-to-noise of 3: 1) are 3.1-18 fmol.
关键词	High Performance Liquid Chromatography-mass Spectrometry Fluorescence Detection Pre-column Derivatization Aliphatic Amines
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	WASTE-WATER ; ACIDS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000247823600001
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6861
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhao Xian-En,Li Yu-Lin,You Jin-Mao,et al. Pre-column derivatization-high performance liquid chromatography for the determination of aliphatic amines with fluorescence detection and mass spectrometric identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(6):779-785.
APA	Zhao Xian-En,Li Yu-Lin,You Jin-Mao,Liu Yong-Jun,&Suo You-Rui.(2007).Pre-column derivatization-high performance liquid chromatography for the determination of aliphatic amines with fluorescence detection and mass spectrometric identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(6),779-785.
MLA	Zhao Xian-En,et al."Pre-column derivatization-high performance liquid chromatography for the determination of aliphatic amines with fluorescence detection and mass spectrometric identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.6(2007):779-785.