Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry

NWIPB OpenIR

	Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry
	Zhu Fang ; Zhao Wen-Chen ; Zhao Xian-En ; Suo You-Rui ; Liu Su-Juan ; You Jin-Mao
	2007-04-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	35 期号:4 页码:489-494
文章类型	Article
摘要	On a reversed-phase Eclipse XDB-C-8 chromatographic column, 20 fatty acids were derivatized using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-d] imidazol-1-yl) ethyl-p-toluenesulfonate (ANITS) as precolumn derivatization reagent and separated by HPLC in conjunction with a gradient elution with fluorescence detection and MS identification. The reagent reacted with fatty acids in the presence of K2CO3 catalyst at 90 degrees C for 40 min in N, N-dimethylformamide solvent to give maximum and constant fluorescence intensity with the addition of 7-fold molar reagent excess to total molar fatty acids. The fluorescence excitation and emission wavelengths of derivatives were lambda(ex) =250 mn and lambda(em) =512 nm, respectively. The identification of fatty acid derivatives was performed by the online APCI/MS (atmospheric pressure chemical ionization source) in positive mode. All linear correlation coefficients for fatty acid derivatives are over 0. 9999, and detection limits, at signal-to-noise of 3:1, are 24. 76 - 98. 79 fmol for labeled fatty acids. The established method is sensitive and repeatable. It has been applied to for the determination of free fatty acids from rape bee pollen samples with satisfactory results.
关键词	High Performance Liquid Chromatography/mass Spectrometry Fluorescence Detection Pre-column Derivatization Fatty Acids Rape Bee Pollen
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	LABELING REAGENT
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000246717800005
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6864
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Zhu Fang,Zhao Wen-Chen,Zhao Xian-En,et al. Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(4):489-494.
APA	Zhu Fang,Zhao Wen-Chen,Zhao Xian-En,Suo You-Rui,Liu Su-Juan,&You Jin-Mao.(2007).Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(4),489-494.
MLA	Zhu Fang,et al."Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.4(2007):489-494.