NWIPB OpenIR
Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization
Ding Yang-Jun; Zhao Xian-En; Zhu Fang; Suo You-Rui; Li Yu-Lin; Chen Cui-Chen; You Jin-Mao
2007-03-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号35期号:3页码:375-381
文章类型Article
摘要A method for the identification of long-chain unsaturated fatty acids in deep-sea fish oil using 1 2-benzo-3, 4-dihydrocarbazole-9-ethyl-benzenesulfonte (BDEBS) as pre-column derivatization reagent by reversed-phase HPLC/MS has been developed. To avoid the migration of double bond in the fatty acid chain, fish oil samples were saponificated and derivatized under nitrogen circumstance. The derivatized solution was extracted with hexane and dried under a stream of nitrogen; the residue was re-dissolved with acetorntrile prior to LC analysis. MS analysis of the fatty acid derivatives exhibited regular information regarding the position of the double bond in the fatty acid chain. With molecular foil mass at m/z [MH](+) and specific fragment ions obtained with MS analysis, the position of the double bond in fatty acid chain was located (or calculated) by means of several established formulas. According to the analysis of HPLC/MS/APCI, 23 fatty acids were identified. The main fatty acids in deep-sea fish oil are C-14-C-22 and 67.08% (area percent) of them are polytinsaturated fatly acids. The main components of the unsaturated fatty acids are C(16:1)9-hexadecenoic acid, 11.7% C16:4 4, 7, 10, 13-hexadecatetraenoic acid, 2.91%; C-18:1 12-octadecenoic acid, 11.1%; C-20:0 6, 9, 12, 15-octadecatetractioic acid, 3.62%; C-20:1 13-eicosenoic acid, 1.21%; C(20:5)5, 8, 11, 14, 17-eicosapentaenoic acid, 16.71%; C(22:6)2, 5, 8, 11, 14, 17-docosahexenoic acid, 10. 53%. The established method provides a new technical tool for the location of doulde bond positions in the, poly unsaturated fatty acids.
关键词High Performance Liquid Chromatography/mass Spectrometry Pre-column Derivatization Fish Oil Fatty Acids Position Of Double Bond
WOS标题词Science & Technology ; Physical Sciences
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000245735200013
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/6867
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式
GB/T 7714
Ding Yang-Jun,Zhao Xian-En,Zhu Fang,et al. Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(3):375-381.
APA Ding Yang-Jun.,Zhao Xian-En.,Zhu Fang.,Suo You-Rui.,Li Yu-Lin.,...&You Jin-Mao.(2007).Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(3),375-381.
MLA Ding Yang-Jun,et al."Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.3(2007):375-381.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ding Yang-Jun]的文章
[Zhao Xian-En]的文章
[Zhu Fang]的文章
百度学术
百度学术中相似的文章
[Ding Yang-Jun]的文章
[Zhao Xian-En]的文章
[Zhu Fang]的文章
必应学术
必应学术中相似的文章
[Ding Yang-Jun]的文章
[Zhao Xian-En]的文章
[Zhu Fang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。